Spatial organization of functional groups on bioactive supramolecular glycopeptide nanofibers for differentiation of mesenchymal stem cells (MSCs) to brown adipogenesis
Author
Caliskan, O. S.
Sardan, Ekiz M.
Tekinay, A. B.
Güler, Mustafa O.
Date
2016-12Source Title
Bioconjugate Chemistry
Print ISSN
1043-1802
Publisher
American Chemical Society
Volume
28
Issue
3
Pages
740 - 750
Language
English
Type
ArticleItem Usage Stats
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Abstract
Spatial organization of bioactive moieties in biological materials has significant impact on the function and efficiency of these systems. Here, we demonstrate the effect of spatial organization of functional groups including carboxylate, amine, and glucose functionalities by using self-assembled peptide amphiphile (PA) nanofibers as a bioactive scaffold. We show that presentation of bioactive groups on glycopeptide nanofibers affects mesenchymal stem cells (MSCs) in a distinct manner by means of adhesion, proliferation, and differentiation. Strikingly, when the glutamic acid is present in the glycopeptide backbone, the PA nanofibers specifically induced differentiation of MSCs into brown adipocytes in the absence of any differentiation medium as shown by lipid droplet accumulation and adipogenic gene marker expression analyses. This effect was not evident in the other glycopeptide nanofibers, which displayed the same functional groups but with different spatial organization. Brown adipocytes are attractive targets for obesity treatment and are found in trace amounts in adults, which also makes this specific glycopeptide nanofiber system an attractive tool to study molecular pathways of brown adipocyte formation.
Keywords
AmineAmphophile
Carboxylic acid
Fat droplet
Functional group
Glucose
Glutamic acid
Glycopeptide
Nanofiber
Biomaterial
Glycopeptide
Nanofiber
Surfactant
Adipogenesis
Article
Brown adipocyte
Cell adhesion
Cell differentiation
Cell proliferation
Cell viability
Gene expression
Marker gene
Mesenchymal stem cell
Obesity
Peptide synthesis
Spatial analysis
Supramolecular chemistry
Animal
Brown adipocyte
Cell culture
Cell line
Chemistry
Cytology
Mesenchymal stroma cell
Metabolism
Rat
Tissue engineering
Tissue scaffold
Ultrastructure
Adipocytes, Brown
Adipogenesis
Animals
Biocompatible Materials
Cell Adhesion
Cell Line
Cell Proliferation
Cells, Cultured
Glycopeptides
Mesenchymal Stromal Cells
Nanofibers
Rats
Surface-Active Agents
Tissue Engineering
Tissue Scaffolds