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      Spatial organization of functional groups on bioactive supramolecular glycopeptide nanofibers for differentiation of mesenchymal stem cells (MSCs) to brown adipogenesis

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      Author(s)
      Caliskan, O. S.
      Sardan, Ekiz M.
      Tekinay, A. B.
      Güler, Mustafa O.
      Date
      2016-12
      Source Title
      Bioconjugate Chemistry
      Print ISSN
      1043-1802
      Publisher
      American Chemical Society
      Volume
      28
      Issue
      3
      Pages
      740 - 750
      Language
      English
      Type
      Article
      Item Usage Stats
      199
      views
      205
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      Abstract
      Spatial organization of bioactive moieties in biological materials has significant impact on the function and efficiency of these systems. Here, we demonstrate the effect of spatial organization of functional groups including carboxylate, amine, and glucose functionalities by using self-assembled peptide amphiphile (PA) nanofibers as a bioactive scaffold. We show that presentation of bioactive groups on glycopeptide nanofibers affects mesenchymal stem cells (MSCs) in a distinct manner by means of adhesion, proliferation, and differentiation. Strikingly, when the glutamic acid is present in the glycopeptide backbone, the PA nanofibers specifically induced differentiation of MSCs into brown adipocytes in the absence of any differentiation medium as shown by lipid droplet accumulation and adipogenic gene marker expression analyses. This effect was not evident in the other glycopeptide nanofibers, which displayed the same functional groups but with different spatial organization. Brown adipocytes are attractive targets for obesity treatment and are found in trace amounts in adults, which also makes this specific glycopeptide nanofiber system an attractive tool to study molecular pathways of brown adipocyte formation.
      Keywords
      Amine
      Amphophile
      Carboxylic acid
      Fat droplet
      Functional group
      Glucose
      Glutamic acid
      Glycopeptide
      Nanofiber
      Biomaterial
      Glycopeptide
      Nanofiber
      Surfactant
      Adipogenesis
      Article
      Brown adipocyte
      Cell adhesion
      Cell differentiation
      Cell proliferation
      Cell viability
      Gene expression
      Marker gene
      Mesenchymal stem cell
      Obesity
      Peptide synthesis
      Spatial analysis
      Supramolecular chemistry
      Animal
      Brown adipocyte
      Cell culture
      Cell line
      Chemistry
      Cytology
      Mesenchymal stroma cell
      Metabolism
      Rat
      Tissue engineering
      Tissue scaffold
      Ultrastructure
      Adipocytes, Brown
      Adipogenesis
      Animals
      Biocompatible Materials
      Cell Adhesion
      Cell Line
      Cell Proliferation
      Cells, Cultured
      Glycopeptides
      Mesenchymal Stromal Cells
      Nanofibers
      Rats
      Surface-Active Agents
      Tissue Engineering
      Tissue Scaffolds
      Permalink
      http://hdl.handle.net/11693/37310
      Published Version (Please cite this version)
      https://doi.org/10.1021/acs.bioconjchem.6b00632
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      • Institute of Materials Science and Nanotechnology (UNAM) 2098
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