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      Supramolecular peptide nanofiber morphology affects mechanotransduction of stem cells

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      Author(s)
      Arslan, Elif
      Koc,, Meryem Hatip
      Uysal, Ozge
      Dikecoglu, Begum
      Topal, Ahmet E.
      Garifullin, Ruslan
      Ozkan, Alper D.
      Dana, A.
      Hermida-Merino, D.
      Castelletto, V.
      Edwards-Gayle, C.
      Baday, S.
      Hamley, I.
      Tekinay, Ayse B.
      Güler, Mustafa O.
      Date
      2017-08
      Source Title
      Biomacromolecules
      Print ISSN
      1525-7797
      Publisher
      American Chemical Society
      Volume
      18
      Issue
      10
      Pages
      3114 - 3130
      Language
      English
      Type
      Article
      Item Usage Stats
      241
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      371
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      Abstract
      Chirality and morphology are essential factors for protein function and interactions with other biomacromolecules. Extracellular matrix (ECM) proteins are also similar to other proteins in this sense; however, the complexity of the natural ECM makes it difficult to study these factors at the cellular level. The synthetic peptide nanomaterials harbor great promise in mimicking specific ECM molecules as model systems. In this work, we demonstrate that mechanosensory responses of stem cells are directly regulated by the chirality and morphology of ECM-mimetic peptide nanofibers with strictly controlled characteristics. Structural signals presented on l-amino acid containing cylindrical nanofibers (l-VV) favored the formation of integrin β1-based focal adhesion complexes, which increased the osteogenic potential of stem cells through the activation of nuclear YAP. On the other hand, twisted ribbon-like nanofibers (l-FF and d-FF) guided the cells into round shapes and decreased the formation of focal adhesion complexes, which resulted in the confinement of YAP proteins in the cytosol and a corresponding decrease in osteogenic potential. Interestingly, the d-form of twisted-ribbon like nanofibers (d-FF) increased the chondrogenic potential of stem cells more than their l-form (l-FF). Our results provide new insights into the importance and relevance of morphology and chirality of nanomaterials in their interactions with cells and reveal that precise control over the chemical and physical properties of nanostructures can affect stem cell fate even without the incorporation of specific epitopes.
      Keywords
      Adhesion
      Biomimetics
      Cells
      Chirality
      Cytology
      Morphology
      Nanofibers
      Nanostructured materials
      Peptides
      Proteins
      Stereochemistry
      Chemical and physical properties
      Chondrogenic potential
      Cylindrical nanofibers
      Extracellular matrix protein
      Mechanotransduction
      Osteogenic potential
      Protein functions
      Synthetic peptide
      Stem cells
      Amino acid
      Amphophile
      Beta1 integrin
      Epitope
      Nanofiber
      Peptide
      Actin capping
      Atomic force microscopy
      Bone development
      Cell adhesion
      Cell proliferation
      Cell viability
      Chirality
      Chondrogenesis
      Confocal microscopy
      Controlled study
      HUVEC cell line
      Permalink
      http://hdl.handle.net/11693/37306
      Published Version (Please cite this version)
      https://doi.org/10.1021/acs.biomac.7b00773
      Collections
      • Aysel Sabuncu Brain Research Center (BAM) 249
      • Institute of Materials Science and Nanotechnology (UNAM) 2260
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