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      Digital holographic microscopy of phase separation in multicomponent lipid membranes

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      Author
      Rad, Vahideh Farzam
      Moradi, Ali-Reza
      Darudi, Ahmad
      Tayebi, Lobat
      Date
      2016
      Source Title
      Journal of Biomedical Optics
      Print ISSN
      1083-3668
      Publisher
      SPIE
      Volume
      21
      Issue
      12
      Language
      English
      Type
      Article
      Item Usage Stats
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      Abstract
      Lateral in-homogeneities in lipid compositions cause microdomains formation and change in the physical properties of biological membranes. With the presence of cholesterol and mixed species of lipids, phospholipid membranes segregate into lateral domains of liquid-ordered and liquid-disordered phases. Coupling of two-dimensional intralayer phase separations and interlayer liquid-crystalline ordering in multicomponent membranes has been previously demonstrated. By the use of digital holographic microscopy (DHMicroscopy), we quantitatively analyzed the volumetric dynamical behavior of such membranes. The specimens are lipid mixtures composed of sphingomyelin, cholesterol, and unsaturated phospholipid, 1,2-dioleoyl-sn-glycero-3-phosphocholine. DHMicroscopy in a transmission mode is an effective tool for quantitative visualization of phase objects. By deriving the associated phase changes, three-dimensional information on the morphology variation of lipid stacks at arbitrary time scales is obtained. Moreover, the thickness distribution of the object at demanded axial planes can be obtained by numerical focusing. Our results show that the volume evolution of lipid domains follows approximately the same universal growth law of previously reported area evolution. However, the thickness of the domains does not alter significantly by time; therefore, the volume evolution is mostly attributed to the changes in area dynamics. These results might be useful in the field of membrane-based functional materials.
      Keywords
      Digital holographic microscopy
      Long range alignment
      Multicomponent lipids
      Phase separation
      Long range alignment
      Permalink
      http://hdl.handle.net/11693/37286
      Published Version (Please cite this version)
      http://dx.doi.org/10.1117/1.JBO.21.12.126016
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