Antigenic GM3 lactone mimetic molecule integrated mannosylated glycopeptide nanofibers for the activation and maturation of dendritic cells
Author
Gunay, Gokhan
Ekiz, Melis Sardan
Ferhati, X.
Richichi, B.
Nativi, C.
Tekinay, Ayse B.
Güler, Mustafa O.
Date
2017Source Title
ACS Applied Materials and Interfaces
Print ISSN
1944-8244
Publisher
American Chemical Society
Volume
9
Issue
19
Pages
16035 - 16042
Language
English
Type
ArticleItem Usage Stats
170
views
views
168
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downloads
Abstract
The ability of dendritic cells to coordinate innate and adaptive immune responses makes them essential targets for vaccination strategies. Presentation of specific antigens by dendritic cells is required for the activation of the immune system against many pathogens and tumors, and nanoscale materials can be functionalized for active targeting of dendritic cells. In this work, we integrated an immunogenic, carbohydrate melanoma-associated antigen-mimetic GM3-lactone molecule into mannosylated peptide amphiphile nanofibers to target dendritic cells through DC-SIGN receptor. Based on morphological and functional analyses, when dendritic cells were treated with peptide nanofiber carriers, they showed significant increase in antigen internalization and a corresponding increase in the surface expression of the activation and maturation markers CD86, CD83 and HLA-DR, in addition to exhibiting a general morphology consistent with dendritic cell maturation. These results indicate that mannosylated peptide amphiphile nanofiber carriers are promising candidates to target dendritic cells for antigen delivery. © 2017 American Chemical Society.
Keywords
Cancer antigensCarbohydrate melanoma associated antigen
Dendritic cells
Amphiphiles
Antigens
Biomimetics
Carbohydrates
Chemical activation
Cytology
Dermatology
Diseases
Esters
Immune system
Molecules
Nanofibers
Oncology
Peptides
Polypeptides
Adaptive immune response
Antigen delivery
Cancer immunotherapy
Dendritic cells
Nano-scale materials
Peptide amphiphiles
Surface expression
Vaccination strategy
Cells
Permalink
http://hdl.handle.net/11693/37285Published Version (Please cite this version)
http://dx.doi.org/10.1021/acsami.7b04094Collections
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