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      Synthetic biogenesis of bacterial amyloid nanomaterials with tunable inorganic-organic interfaces and electrical conductivity

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      Author(s)
      Seker U.O.S.
      Chen, A. Y.
      Citorik, R. J.
      Lu, T. K.
      Date
      2017
      Source Title
      ACS Synthetic Biology
      Print ISSN
      2161-5063
      Publisher
      American Chemical Society
      Volume
      6
      Issue
      2
      Pages
      266 - 275
      Language
      English
      Type
      Article
      Item Usage Stats
      237
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      491
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      Abstract
      Amyloids are highly ordered, hierarchal protein nanoassemblies. Functional amyloids in bacterial biofilms, such as Escherichia coli curli fibers, are formed by the polymerization of monomeric proteins secreted into the extracellular space. Curli is synthesized by living cells, is primarily composed of the major curlin subunit CsgA, and forms biological nanofibers with high aspect ratios. Here, we explore the application of curli fibers for nanotechnology by engineering curli to mediate tunable biological interfaces with inorganic materials and to controllably form gold nanoparticles and gold nanowires. Specifically, we used cell-synthesized curli fibers as templates for nucleating and growing gold nanoparticles and showed that nanoparticle size could be modulated as a function of curli fiber gold-binding affinity. Furthermore, we demonstrated that gold nanoparticles can be preseeded onto curli fibers and followed by gold enhancement to form nanowires. Using these two approaches, we created artificial cellular systems that integrate inorganic-organic materials to achieve tunable electrical conductivity. We envision that cell-synthesized amyloid nanofibers will be useful for interfacing abiotic and biotic systems to create living functional materials.
      Keywords
      Biofilm proteins
      Functional amyloids
      Living materials
      Nanomaterial assembly
      Amyloid
      Bacterial protein
      Gold nanoparticle
      Gold nanowire
      Inorganic compound
      Nanomaterial
      Nanowire
      Organic compound
      Unclassified drug
      Amyloid
      Bacterial protein
      Crl protein, Bacteria
      Escherichia coli protein
      Gold
      Metal nanoparticle
      Nanofiber
      Nanomaterial
      Article
      Binding affinity
      Biogenesis
      Chemical engineering
      Electric conductivity
      Escherichia coli
      Metal binding
      Nanotechnology
      Nonhuman
      Particle size
      Priority journal
      Protein assembly
      Protein secondary structure
      Transmission electron microscopy
      Biofilm
      Electric conductivity
      Growth, development and aging
      Metabolism
      Microbiology
      Procedures
      Amyloid
      Bacterial Proteins
      Biofilms
      Electric Conductivity
      Escherichia coli
      Escherichia coli Proteins
      Gold
      Metal Nanoparticles
      Nanofibers
      Nanostructures
      Nanotechnology
      Nanowires
      Particle Size
      Permalink
      http://hdl.handle.net/11693/37274
      Published Version (Please cite this version)
      https://doi.org/10.1021/acssynbio.6b00166
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      • Institute of Materials Science and Nanotechnology (UNAM) 2260
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