Local delivery of doxorubicin through supramolecular peptide amphiphile nanofiber gels
Date
2017Source Title
Biomaterials Science
Print ISSN
2047-4830
Publisher
Royal Society of Chemistry
Volume
5
Issue
1
Pages
67 - 76
Language
English
Type
ArticleItem Usage Stats
204
views
views
502
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downloads
Abstract
Peptide amphiphiles (PAs) self-assemble into supramolecular nanofiber gels that provide a suitable environment for encapsulation of both hydrophobic and hydrophilic molecules. The PA gels have significant advantages for controlled delivery applications due to their high capacity to retain water, biocompatibility, and biodegradability. In this study, we demonstrate injectable supramolecular PA nanofiber gels for drug delivery applications. Doxorubicin (Dox), as a widely used chemotherapeutic drug for breast cancer treatment, was encapsulated within the PA gels prepared at different concentrations. Physical and chemical properties of the gels were characterized, and slow release of the Dox molecules through the supramolecular PA nanofiber gels was studied. In addition, the diffusion constants of the drug molecules within the PA nanofiber gels were estimated using fluorescence recovery after the photobleaching (FRAP) method. The PA nanofiber gels did not show any cytotoxicity and the encapsulation strategy enhanced the activity of drug molecules on cellular viability through prolonged release compared to direct administration under in vitro conditions. Moreover, the local in vivo injection of the Dox encapsulated PA nanofiber gels (Dox/PA) to the tumor site demonstrated the lowest tumor growth rate compared to the direct Dox injection and increased the apoptotic cells within the tumor tissue for local drug release through the PA nanofiber gels under in vivo conditions.
Keywords
AmphiphilesBiocompatibility
Biodegradability
Molecules
Nanofibers
Peptides
Photobleaching
Polypeptides
Supramolecular chemistry
Tumors
Breast cancer treatment
Chemotherapeutic drugs
Controlled delivery
Drug delivery applications
Fluorescence recovery
Hydrophobic and hydrophilic
Peptide amphiphiles
Physical and chemical properties
Gels
Amphophile
Doxorubicin
Nanofiber
Peptide
Doxorubicin
Nanofiber
Peptide
Surfactant
Animal experiment
Animal model
Animal tissue
Article
Bleaching
Breast cancer cell line
Breast carcinoma
Cell viability
Concentration (parameters)
Controlled study
Cytotoxicity
Drug delivery system
Drug release
Female
Mouse
Nanoencapsulation
Nonhuman
Physical chemistry
Priority journal
Breast tumor
Chemical phenomena
Permalink
http://hdl.handle.net/11693/37254Published Version (Please cite this version)
https://doi.org/10.1039/c6bm00656fCollections
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