Bioactive peptide functionalized aligned cyclodextrin nanofibers for neurite outgrowth
Date
2017Source Title
Journal of Materials Chemistry B
Print ISSN
2050-7518
Publisher
Royal Society of Chemistry
Volume
5
Issue
3
Pages
517 - 524
Language
English
Type
ArticleItem Usage Stats
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Abstract
Guidance of neurite extension and establishment of neural connectivity hold great importance for neural tissue regeneration and neural conduit implants. Although bioactive-epitope functionalized synthetic or natural polymeric materials have been proposed for the induction of neural regeneration, chemical modifications of these materials for neural differentiation still remain a challenge due to the harsh conditions of chemical reactions, along with non-homogeneous surface modifications. In this study, a facile noncovalent functionalization method is proposed by exploiting host-guest interactions between an adamantane-conjugated laminin derived bioactive IKVAV epitope and electrospun cyclodextrin nanofibers (CDNFs) to fabricate implantable scaffolds for peripheral nerve regeneration. While electrospun CDNFs introduce a three-dimensional biocompatible microenvironment to promote cellular viability and adhesion, the bioactive epitopes presented on the surface of electrospun CDNFs guide the cellular differentiation of PC-12 cells. In addition to materials synthesis and smart functionalization, physical alignment of the electrospun nanofibers guides the cells for enhanced differentiation. Cells cultured on aligned and IKVAV functionalized electrospun CDNFs had significantly higher expression of neuron-specific βIII-tubulin and synaptophysin. The neurite extension is also higher on the bioactive aligned scaffolds compared to random and non-functionalized electrospun CDNFs. Both chemical and physical cues were utilized for an effective neuronal differentiation process. © The Royal Society of Chemistry.
Keywords
BiocompatibilityChemical modification
Cyclodextrins
Epitopes
Nanofibers
Neurons
Surface treatment
Cellular differentiation
Enhanced differentiations
Host guest interactions
Neural differentiations
Neural tissue regeneration
Neuronal differentiation
Non-covalent functionalization
Peripheral nerve regeneration
Tissue regeneration