• About
  • Policies
  • What is open access
  • Library
  • Contact
Advanced search
      View Item 
      •   BUIR Home
      • Scholarly Publications
      • Institute of Materials Science and Nanotechnology (UNAM)
      • View Item
      •   BUIR Home
      • Scholarly Publications
      • Institute of Materials Science and Nanotechnology (UNAM)
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      Bioactive peptide functionalized aligned cyclodextrin nanofibers for neurite outgrowth

      Thumbnail
      View / Download
      5.1 Mb
      Author(s)
      Hamsici, S.
      Cinar, G.
      Celebioglu A.
      Uyar, Tamer
      Tekinay, A. B.
      Güler, Mustafa O.
      Date
      2017
      Source Title
      Journal of Materials Chemistry B
      Print ISSN
      2050-7518
      Publisher
      Royal Society of Chemistry
      Volume
      5
      Issue
      3
      Pages
      517 - 524
      Language
      English
      Type
      Article
      Item Usage Stats
      253
      views
      383
      downloads
      Abstract
      Guidance of neurite extension and establishment of neural connectivity hold great importance for neural tissue regeneration and neural conduit implants. Although bioactive-epitope functionalized synthetic or natural polymeric materials have been proposed for the induction of neural regeneration, chemical modifications of these materials for neural differentiation still remain a challenge due to the harsh conditions of chemical reactions, along with non-homogeneous surface modifications. In this study, a facile noncovalent functionalization method is proposed by exploiting host-guest interactions between an adamantane-conjugated laminin derived bioactive IKVAV epitope and electrospun cyclodextrin nanofibers (CDNFs) to fabricate implantable scaffolds for peripheral nerve regeneration. While electrospun CDNFs introduce a three-dimensional biocompatible microenvironment to promote cellular viability and adhesion, the bioactive epitopes presented on the surface of electrospun CDNFs guide the cellular differentiation of PC-12 cells. In addition to materials synthesis and smart functionalization, physical alignment of the electrospun nanofibers guides the cells for enhanced differentiation. Cells cultured on aligned and IKVAV functionalized electrospun CDNFs had significantly higher expression of neuron-specific βIII-tubulin and synaptophysin. The neurite extension is also higher on the bioactive aligned scaffolds compared to random and non-functionalized electrospun CDNFs. Both chemical and physical cues were utilized for an effective neuronal differentiation process. © The Royal Society of Chemistry.
      Keywords
      Biocompatibility
      Chemical modification
      Cyclodextrins
      Epitopes
      Nanofibers
      Neurons
      Surface treatment
      Cellular differentiation
      Enhanced differentiations
      Host guest interactions
      Neural differentiations
      Neural tissue regeneration
      Neuronal differentiation
      Non-covalent functionalization
      Peripheral nerve regeneration
      Tissue regeneration
      Permalink
      http://hdl.handle.net/11693/37246
      Published Version (Please cite this version)
      https://doi.org/10.1039/c6tb02441f
      Collections
      • Institute of Materials Science and Nanotechnology (UNAM) 2260
      Show full item record

      Browse

      All of BUIRCommunities & CollectionsTitlesAuthorsAdvisorsBy Issue DateKeywordsTypeDepartmentsCoursesThis CollectionTitlesAuthorsAdvisorsBy Issue DateKeywordsTypeDepartmentsCourses

      My Account

      Login

      Statistics

      View Usage StatisticsView Google Analytics Statistics

      Bilkent University

      If you have trouble accessing this page and need to request an alternate format, contact the User and Access Services. Phone: (312) 290 1298
      © Bilkent University - Library IT

      Contact Us | Send Feedback | Off-Campus Access | Admin | Privacy