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      Genetically encoded conductive protein nanofibers secreted by engineered cells

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      Author(s)
      Kalyoncu, E.
      Ahan, R. E.
      Olmez, T. T.
      Safak Seker, U. O.
      Date
      2017-06
      Source Title
      RSC Advances
      Print ISSN
      2046-2069
      Publisher
      Royal Society of Chemistry
      Volume
      7
      Issue
      52
      Pages
      32543 - 32551
      Language
      English
      Type
      Article
      Item Usage Stats
      197
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      163
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      Abstract
      Bacterial biofilms are promising tools for functional applications as bionanomaterials. They are synthesized by well-defined machinery, readily form fiber networks covering large areas, and can be engineered for different functionalities. In this work, bacterial biofilms have been engineered for use as conductive biopolymers to interface with electrodes and connect bacterial populations to electronic gadgets. Bacterial biofilms are designed with different conductive peptide motifs, as the aromatic amino acid content of fused peptide motifs has been suggested to contribute to electronic conductivity by influencing monomer stacking behavior. To select the best candidates for constructing conductive peptide motifs, conductivity properties of aromatic amino acids are measured using two different fiber scaffolds, an amyloid-like fiber (ALF) forming peptide, and the amyloidogenic R5T peptide of CsgA protein. Three repeats of aromatic amino acids are added to fiber-forming peptide sequences to produce delocalized π clouds similar to those observed in conductive polymers. Based on the measurements, tyrosine and tryptophan residues provide the highest conductivity. Therefore, the non-conductive E. coli biofilm is switched into a conductive form by genetically inserted conductive peptide motifs containing different combinations of tyrosine and tryptophan. Finally, synthetic biofilm biogenesis is achieved with conductive peptide motifs using controlled biofilm production. Conductive biofilms on living cells are formed for bioelectronics and biosensing applications.
      Keywords
      Amino acids
      Aromatic compounds
      Aromatic polymers
      Aromatization
      Biofilms
      Biopolymers
      Escherichia coli
      Fibers
      Machinery
      Proteins
      Scaffolds (biology)
      Aromatic amino acid
      Bacterial population
      Biosensing applications
      Conductive biofilms
      Conductivity properties
      Electronic conductivity
      Functional applications
      Tryptophan residues
      Peptides
      Permalink
      http://hdl.handle.net/11693/37235
      Published Version (Please cite this version)
      https://doi.org/10.1039/c7ra06289c
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      • Institute of Materials Science and Nanotechnology (UNAM) 2258
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