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      Circulating LL37 targets plasma extracellular vesicles to immune cells and intensifies Behçet's disease severity

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      Author(s)
      Kahraman, T.
      Gucluler, G.
      Simsek, I.
      Yagci, F. C.
      Yildirim, M.
      Ozen, C.
      Dinc, A.
      Gursel, M.
      Ikromzoda, L.
      Sutlu, T.
      Gay, S.
      Gursel, I.
      Date
      2017-02
      Source Title
      Journal of Extracellular Vesicles
      Print ISSN
      2001-3078
      Publisher
      Taylor and Francis
      Volume
      6
      Issue
      1
      Pages
      1284449-1 - 1284449-13
      Language
      English
      Type
      Article
      Item Usage Stats
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      204
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      Abstract
      Behçet's disease (BD) activity is characterised by sustained, over-exuberant immune activation, yet the underlying mechanisms leading to active BD state are poorly defined. Herein, we show that the human cathelicidin derived antimicrobial peptide LL37 associates with and directs plasma extracellular vesicles (EV) to immune cells, thereby leading to enhanced immune activation aggravating BD pathology. Notably, disease activity was correlated with elevated levels of circulating LL37 and EV plasma concentration. Stimulation of healthy PBMC with active BD patient EVs induced heightened IL1β, IFNα, IL6 and IP10 secretion compared to healthy and inactive BD EVs. Remarkably, when mixed with LL37, healthy plasma-EVs triggered a robust immune activation replicating the pathology inducing properties of BD EVs. The findings of this study could be of clinical interest in the management of BD, implicating LL37/EV association as one of the major contributors of BD pathogenesis.
      Keywords
      Autoimmune
      Behçet's disease
      Cytokine
      Extracellular vesicles
      Immune activation
      LL37
      Alpha interferon
      Biological marker
      Cathelicidin
      Cathelicidin antimicrobial peptide LL 37
      Gamma interferon inducible protein 10
      Glucose regulated protein 94
      Interleukin 10
      Interleukin 1beta
      Interleukin 6
      Protein Alix
      Unclassified drug
      Allogeneic extracellular vesicle
      Article
      Autologous extracellular vesicle
      Behcet disease
      Cell stimulation
      Comparative study
      Complex formation
      Controlled study
      Cytokine production
      Cytokine release
      Disease activity
      Disease association
      Disease severity
      Exosome
      Human
      Human cell
      Immune activation
      Immune response
      Immunocompetent cell
      Incubation time
      Internalization
      Pathogenesis
      Peripheral blood mononuclear cell
      Plasma extracellular vesicle
      Plasmacytoid dendritic cell
      Permalink
      http://hdl.handle.net/11693/37177
      Published Version (Please cite this version)
      https://doi.org/10.1080/20013078.2017.1284449
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      • Department of Molecular Biology and Genetics 512
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