• About
  • Policies
  • What is open access
  • Library
  • Contact
Advanced search
      View Item 
      •   BUIR Home
      • Scholarly Publications
      • Institute of Materials Science and Nanotechnology (UNAM)
      • View Item
      •   BUIR Home
      • Scholarly Publications
      • Institute of Materials Science and Nanotechnology (UNAM)
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      Toxicity of internalized laser generated pure silver nanoparticles to the isolated rat hippocampus cells

      Thumbnail
      View / Download
      430.5 Kb
      Author(s)
      Kursungoz, C.
      Taş, S. T.
      Sargon, M. F.
      Sara, Y.
      Ortaç, B.
      Date
      2017-02
      Source Title
      Toxicology and Industrial Health
      Print ISSN
      0748-2337
      Publisher
      SAGE
      Volume
      33
      Issue
      7
      Pages
      555 - 563
      Language
      English
      Type
      Article
      Item Usage Stats
      206
      views
      267
      downloads
      Abstract
      Silver nanoparticles (AgNPs) are the most commonly used nanoparticles (NPs) in medicine, industry and cosmetics. They are generally considered as biocompatible. However, contradictory reports on their biosafety render them difficult to accept as 'safe'. In this study, we evaluated the neurotoxicity of direct AgNP treatment in rat hippocampal slices. We produced pure uncoated AgNPs by a pulsed laser ablation method. NP characterization was performed by Ultraviolet (UV) visible spectrophotometer, scanning electron microscope, transmission electron microscope (TEM) and energy-dispersive X-ray spectroscopy. Rat hippocampal slices were treated with AgNPs for an hour. AgNP exposure of hippocampal tissue resulted in a significant decrease in cell survival in a dose-dependent manner. Our TEM results showed that AgNPs were distributed in the extracellular matrix and were taken into the cytoplasm of the neurons. Moreover, we found that only larger AgNPs were taken into the neurons via phagocytosis. This study showed that the pure AgNPs produced by laser ablation are toxic to the neural tissue. We also found that neurons internalized only the large NPs by phagocytosis which seems to be the major mechanism in AgNP neurotoxicity.
      Keywords
      Classical endocytosis
      Hippocampus
      Internalization of nanoparticles
      Laser ablation
      Neurodegenerative disease
      Silver nanoparticles
      Silver nanoparticle
      Animal tissue
      Article
      Brain nerve cell
      Cell survival
      Cell viability
      Controlled study
      Cytoplasm
      Cytotoxicity
      Electron microscope
      Energy dispersive X ray spectroscopy
      Extracellular matrix
      Hippocampal slice
      Hippocampal tissue
      Hippocampus
      Male
      Nanotoxicology
      Neodymium YLF laser
      Neurotoxicity
      Nonhuman
      Phagocytosis
      Rat
      Scanning electron microscope
      Scanning electron microscopy
      Transmission electron microscopy
      Ultraviolet spectrophotometer
      Ultraviolet spectrophotometry
      Permalink
      http://hdl.handle.net/11693/37056
      Published Version (Please cite this version)
      https://doi.org/10.1177/0748233717690992
      Collections
      • Institute of Materials Science and Nanotechnology (UNAM) 2260
      Show full item record

      Browse

      All of BUIRCommunities & CollectionsTitlesAuthorsAdvisorsBy Issue DateKeywordsTypeDepartmentsCoursesThis CollectionTitlesAuthorsAdvisorsBy Issue DateKeywordsTypeDepartmentsCourses

      My Account

      Login

      Statistics

      View Usage StatisticsView Google Analytics Statistics

      Bilkent University

      If you have trouble accessing this page and need to request an alternate format, contact the User and Access Services. Phone: (312) 290 1298
      © Bilkent University - Library IT

      Contact Us | Send Feedback | Off-Campus Access | Admin | Privacy