MicroRNA expression patterns in canine mammary cancer show significant differences between metastatic and non-metastatic tumours
Author(s)
Date
2017Source Title
BMC Cancer
Print ISSN
1471-2407
Publisher
BioMed Central Ltd.
Volume
17
Issue
728
Pages
1 - 17
Language
English
Type
ArticleItem Usage Stats
225
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views
171
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downloads
Abstract
Background: MicroRNAs may act as oncogenes or tumour suppressor genes, which make these small molecules potential diagnostic/prognostic factors and targets for anticancer therapies. Several common oncogenic microRNAs have been found for canine mammary cancer and human breast cancer. On account of this, large-scale profiling of microRNA expression in canine mammary cancer seems to be important for both dogs and humans. Methods: Expression profiles of 317 microRNAs in 146 canine mammary tumours of different histological type, malignancy grade and clinical history (presence/absence of metastases) and in 25 control samples were evaluated. The profiling was performed using microarrays. Significance Analysis of Microarrays test was applied in the analysis of microarray data (both unsupervised and supervised data analyses were performed). Validation of the obtained results was performed using real-time qPCR. Subsequently, predicted targets for the microRNAs were searched for in miRBase. Results: Results of the unsupervised analysis indicate that the primary factor separating the samples is the metastasis status. Predicted targets for microRNAs differentially expressed in the metastatic vs. non-metastatic group are mostly engaged in cell cycle regulation, cell differentiation and DNA-damage repair. On the other hand, the supervised analysis reveals clusters of differentially expressed microRNAs unique for the tumour type, malignancy grade and metastasis factor. Conclusions: The most significant difference in microRNA expression was observed between the metastatic and non-metastatic group, which suggests a more important role of microRNAs in the metastasis process than in the malignant transformation. Moreover, the differentially expressed microRNAs constitute potential metastasis markers. However, validation of cfa-miR-144, cfa-miR-32 and cfa-miR-374a levels in blood samples did not follow changes observed in the non-metastatic and metastatic tumours.
Keywords
Canine mammary cancerHuman breast cancer
MicroRNA
cfa miR 144
cfa miR 32
cfa miR 374a
MicroRNA
Unclassified drug
Animal tissue
Breast cancer
Cancer grading
Cell cycle regulation
Cell differentiation
Controlled study
DNA repair
Dog
Female
Gene expression
Metastasis
Microarray analysis
Nonhuman
Real time polymerase chain reaction
Tumor classification
Permalink
http://hdl.handle.net/11693/37047Published Version (Please cite this version)
https://doi.org/10.1186/s12885-017-3751-1Collections
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