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dc.contributor.authorAakhte, M.en_US
dc.contributor.authorAbbasian, V.en_US
dc.contributor.authorAkhlaghi, E. A.en_US
dc.contributor.authorMoradi, A. R.en_US
dc.contributor.authorAnand, A.en_US
dc.contributor.authorJavidi, B.en_US
dc.date.accessioned2018-04-12T11:01:01Z
dc.date.available2018-04-12T11:01:01Z
dc.date.issued2017en_US
dc.identifier.issn1559-128X
dc.identifier.urihttp://hdl.handle.net/11693/37039
dc.description.abstractIn this paper, we use a glass microsphere incorporated into a digital holographic microscope to increase the effective resolution of the system, aiming at precise cell identification. A Mirau interferometric objective is employed in the experiments, which can be used for a common-path digital holographic microscopy (DHMicroscopy) arrangement. High-magnification Mirau objectives are expensive and suffer from low working distances, yet the commonly used low-magnification Mirau objectives do not have high lateral resolutions. We show that by placing a glass microsphere within the working distance of a low-magnification Mirau objective, its effective numerical aperture can be increased, leading to super-resolved three-dimensional images. The improvement in the lateral resolution depends on the size and vertical position of microsphere, and by varying these parameters, the lateral resolution and magnification may be adjusted. We used the information from the super-resolution DHMicroscopy to identify thalassemia minor red blood cells (tRBCs). Identification is done by comparing the volumetric measurements with those of healthy RBCs. Our results show that microsphere-assisted super-resolved Mirau DHMicroscopy, being common path and off-axis in nature, has the potential to serve as a benchtop device for cell identification and biomedical measurements.en_US
dc.language.isoEnglishen_US
dc.source.titleApplied Opticsen_US
dc.relation.isversionofhttp://dx.doi.org/10.1364/AO.56.0000D8en_US
dc.subjectCellsen_US
dc.subjectCytologyen_US
dc.subjectGlassen_US
dc.subjectHolographyen_US
dc.subjectMicroscopic examinationen_US
dc.subjectPhase measurementen_US
dc.subjectBiomedical measurementsen_US
dc.subjectDigital holographic microscopyen_US
dc.subjectEffective numerical apertureen_US
dc.subjectEffective resolutionsen_US
dc.subjectHigh magnificationsen_US
dc.subjectHigh-lateral resolutionen_US
dc.subjectThree dimensional imagesen_US
dc.subjectVolumetric measurementen_US
dc.subjectMicrospheresen_US
dc.titleMicrosphere-Assisted Super-Resolved Mirau Digital Holographic Microscopy for Cell Identificationen_US
dc.typeArticleen_US
dc.departmentDepartment of Physicsen_US
dc.citation.spageD8en_US
dc.citation.epageD13en_US
dc.citation.volumeNumber56en_US
dc.citation.issueNumber9en_US
dc.identifier.doi10.1364/AO.56.0000D8en_US
dc.publisherOSA - The Optical Societyen_US


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