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      Microsphere-Assisted Super-Resolved Mirau Digital Holographic Microscopy for Cell Identification

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      Author(s)
      Aakhte, M.
      Abbasian, V.
      Akhlaghi, E. A.
      Moradi, A. R.
      Anand, A.
      Javidi, B.
      Date
      2017
      Source Title
      Applied Optics
      Print ISSN
      1559-128X
      Publisher
      OSA - The Optical Society
      Volume
      56
      Issue
      9
      Pages
      D8 - D13
      Language
      English
      Type
      Article
      Item Usage Stats
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      495
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      Abstract
      In this paper, we use a glass microsphere incorporated into a digital holographic microscope to increase the effective resolution of the system, aiming at precise cell identification. A Mirau interferometric objective is employed in the experiments, which can be used for a common-path digital holographic microscopy (DHMicroscopy) arrangement. High-magnification Mirau objectives are expensive and suffer from low working distances, yet the commonly used low-magnification Mirau objectives do not have high lateral resolutions. We show that by placing a glass microsphere within the working distance of a low-magnification Mirau objective, its effective numerical aperture can be increased, leading to super-resolved three-dimensional images. The improvement in the lateral resolution depends on the size and vertical position of microsphere, and by varying these parameters, the lateral resolution and magnification may be adjusted. We used the information from the super-resolution DHMicroscopy to identify thalassemia minor red blood cells (tRBCs). Identification is done by comparing the volumetric measurements with those of healthy RBCs. Our results show that microsphere-assisted super-resolved Mirau DHMicroscopy, being common path and off-axis in nature, has the potential to serve as a benchtop device for cell identification and biomedical measurements.
      Keywords
      Cells
      Cytology
      Glass
      Holography
      Microscopic examination
      Phase measurement
      Biomedical measurements
      Digital holographic microscopy
      Effective numerical aperture
      Effective resolutions
      High magnifications
      High-lateral resolution
      Three dimensional images
      Volumetric measurement
      Microspheres
      Permalink
      http://hdl.handle.net/11693/37039
      Published Version (Please cite this version)
      http://dx.doi.org/10.1364/AO.56.0000D8
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