Microsphere-Assisted Super-Resolved Mirau Digital Holographic Microscopy for Cell Identification
Date
2017Source Title
Applied Optics
Print ISSN
1559-128X
Publisher
OSA - The Optical Society
Volume
56
Issue
9
Pages
D8 - D13
Language
English
Type
ArticleItem Usage Stats
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Abstract
In this paper, we use a glass microsphere incorporated into a digital holographic microscope to increase the effective resolution of the system, aiming at precise cell identification. A Mirau interferometric objective is employed in the experiments, which can be used for a common-path digital holographic microscopy (DHMicroscopy) arrangement. High-magnification Mirau objectives are expensive and suffer from low working distances, yet the commonly used low-magnification Mirau objectives do not have high lateral resolutions. We show that by placing a glass microsphere within the working distance of a low-magnification Mirau objective, its effective numerical aperture can be increased, leading to super-resolved three-dimensional images. The improvement in the lateral resolution depends on the size and vertical position of microsphere, and by varying these parameters, the lateral resolution and magnification may be adjusted. We used the information from the super-resolution DHMicroscopy to identify thalassemia minor red blood cells (tRBCs). Identification is done by comparing the volumetric measurements with those of healthy RBCs. Our results show that microsphere-assisted super-resolved Mirau DHMicroscopy, being common path and off-axis in nature, has the potential to serve as a benchtop device for cell identification and biomedical measurements.
Keywords
CellsCytology
Glass
Holography
Microscopic examination
Phase measurement
Biomedical measurements
Digital holographic microscopy
Effective numerical aperture
Effective resolutions
High magnifications
High-lateral resolution
Three dimensional images
Volumetric measurement
Microspheres