Ultrasensitive electrospun fluorescent nanofibrous membrane for rapid visual colorimetric detection of H2O2
Date
2016-02Source Title
Analytical and Bioanalytical Chemistry
Print ISSN
1618-2642
Publisher
Springer Verlag
Volume
408
Issue
5
Pages
1347 - 1355
Language
English
Type
ArticleItem Usage Stats
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Abstract
We report herein a flexible fluorescent nanofibrous membrane (FNFM) prepared by decorating the gold nanocluster (AuNC) on electrospun polysulfone nanofibrous membrane for rapid visual colorimetric detection of H2O2. The provision of AuNC coupled to NFM has proven to be advantageous for facile and quick visualization of the obtained results, permitting instant, selective, and on-site detection. We strongly suggest that the fast response time is ascribed to the enhanced probabilities of interaction with AuNC located at the surface of NF. It has been observed that the color change from red to blue is dependent on the concentration, which is exclusively selective for hydrogen peroxide. The detection limit has been found to be 500 nM using confocal laser scanning microscope (CLSM), visually recognizable with good accuracy and stability. A systematic comparison was performed between the sensing performance of FNFM and AuNC solution. The underlying sensing mechanism is demonstrated using UV spectra, transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). The corresponding disappearance of the characteristic emissions of gold nanoclusters and the emergence of a localized surface plasmon resonance (LSPR) band, stressing this unique characteristic of gold nanoparticles. Hence, it is evident that the conversion of nanoparticles from nanoclusters has taken place in the presence of H2O2. Our work here has paved a new path for the detection of bioanalytes, highlighting the merits of rapid readout, sensitivity, and user-friendliness.
Keywords
FluorescenceGold nanocluster
Hydrogen peroxide
Electrospinning
Nanofiber
Sensor
Color
Colorimetry
Electrospinning
Fluorescence
Gold
High resolution transmission electron microscopy
Hydrogen peroxide
Multilayers
Nanoclusters
Nanofibers
Nanoparticles
Oxidation
Peroxides
Photoelectron spectroscopy
Sensors
Surface plasmon resonance
Transmission electron microscopy
Ultraviolet spectroscopy
Characteristic emission
Confocal laser scanning microscope
Gold nanocluster
Gold Nanoparticles
Localized surface plasmon resonance
Nanofibrous membranes
Sensing performance
Visual colorimetric detections
X ray photoelectron spectroscopy
artificial membrane
Gold
Hydrogen peroxide
Metal nanoparticle
Nanofiber
Artificial membrane
Chemistry
Devices
Electrochemical analysis
Genetic procedures
Infrared spectroscopy
Surface plasmon resonance
Transmission electron microscopy
X ray photoelectron spectroscopy
Biosensing Techniques
Electrochemical Techniques
Gold
Hydrogen Peroxide
Membranes, Artificial
Metal Nanoparticles
Microscopy, Electron, Transmission
Nanofibers
Photoelectron Spectroscopy
Spectroscopy, Fourier Transform Infrared
Surface Plasmon Resonance
Permalink
http://hdl.handle.net/11693/36935Published Version (Please cite this version)
https://doi.org/10.1007/s00216-015-9149-5Collections
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