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      Protein-releasing conductive anodized alumina membranes for nerve-interface materials

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      Author
      Altuntas, S.
      Buyukserin, F.
      Haider, A.
      Altinok, B.
      Bıyıklı, Necmi
      Aslim, B.
      Date
      2016
      Source Title
      Materials Science and Engineering C: Materials for Biological Applications
      Print ISSN
      0928-4931
      Publisher
      Elsevier Ltd
      Volume
      67
      Pages
      590 - 598
      Language
      English
      Type
      Article
      Item Usage Stats
      140
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      128
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      Abstract
      Nanoporous anodized alumina membranes (AAMs) have numerous biomedical applications spanning from biosensors to controlled drug delivery and implant coatings. Although the use of AAM as an alternative bone implant surface has been successful, its potential as a neural implant coating remains unclear. Here, we introduce conductive and nerve growth factor-releasing AAM substrates that not only provide the native nanoporous morphology for cell adhesion, but also induce neural differentiation. We recently reported the fabrication of such conductive membranes by coating AAMs with a thin C layer. In this study, we investigated the influence of electrical stimulus, surface topography, and chemistry on cell adhesion, neurite extension, and density by using PC 12 pheochromocytoma cells in a custom-made glass microwell setup. The conductive AAMs showed enhanced neurite extension and generation with the electrical stimulus, but cell adhesion on these substrates was poorer compared to the naked AAMs. The latter nanoporous material presents chemical and topographical features for superior neuronal cell adhesion, but, more importantly, when loaded with nerve growth factor, it can provide neurite extension similar to an electrically stimulated CAAM counterpart.
      Keywords
      Alumina membranes
      Biomaterials
      Electrical stimulation
      Nanotechnology
      PC12 cells
      Alumina
      Biomaterials
      Cell adhesion
      Cells
      Coatings
      Controlled drug delivery
      Cytology
      Interfaces (materials)
      Medical applications
      Membranes
      Nanopores
      Nanotechnology
      Neurons
      Porous materials
      Surface topography
      Alumina membranes
      Anodized alumina membranes
      Biomedical applications
      Electrical stimulations
      Nanoporous morphologies
      Neural differentiations
      PC-12 cells
      PC-12 pheochromocytoma cells
      Conductive materials
      Aluminum oxide
      Artificial membrane
      Delayed release formulation
      Nerve growth factor
      Animal
      Artificial membrane
      Cell adhesion
      Chemistry
      Delayed release formulation
      Drug effects
      Electric conductivity
      PC12 cell line
      Pharmacokinetics
      Pharmacology
      Rat
      Aluminum oxide
      Animals
      Cell adhesion
      Delayed-action preparations
      Electric conductivity
      Membranes, artificial
      Nerve growth factor
      PC12 cells
      Rats
      Permalink
      http://hdl.handle.net/11693/36803
      Published Version (Please cite this version)
      http://dx.doi.org/10.1016/j.msec.2016.05.084
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      • Institute of Materials Science and Nanotechnology (UNAM) 1775
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