Protein-releasing conductive anodized alumina membranes for nerve-interface materials
Author
Altuntas, S.
Buyukserin, F.
Haider, A.
Altinok, B.
Bıyıklı, Necmi
Aslim, B.
Date
2016Source Title
Materials Science and Engineering C: Materials for Biological Applications
Print ISSN
0928-4931
Publisher
Elsevier Ltd
Volume
67
Pages
590 - 598
Language
English
Type
ArticleItem Usage Stats
140
views
views
128
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downloads
Abstract
Nanoporous anodized alumina membranes (AAMs) have numerous biomedical applications spanning from biosensors to controlled drug delivery and implant coatings. Although the use of AAM as an alternative bone implant surface has been successful, its potential as a neural implant coating remains unclear. Here, we introduce conductive and nerve growth factor-releasing AAM substrates that not only provide the native nanoporous morphology for cell adhesion, but also induce neural differentiation. We recently reported the fabrication of such conductive membranes by coating AAMs with a thin C layer. In this study, we investigated the influence of electrical stimulus, surface topography, and chemistry on cell adhesion, neurite extension, and density by using PC 12 pheochromocytoma cells in a custom-made glass microwell setup. The conductive AAMs showed enhanced neurite extension and generation with the electrical stimulus, but cell adhesion on these substrates was poorer compared to the naked AAMs. The latter nanoporous material presents chemical and topographical features for superior neuronal cell adhesion, but, more importantly, when loaded with nerve growth factor, it can provide neurite extension similar to an electrically stimulated CAAM counterpart.
Keywords
Alumina membranesBiomaterials
Electrical stimulation
Nanotechnology
PC12 cells
Alumina
Biomaterials
Cell adhesion
Cells
Coatings
Controlled drug delivery
Cytology
Interfaces (materials)
Medical applications
Membranes
Nanopores
Nanotechnology
Neurons
Porous materials
Surface topography
Alumina membranes
Anodized alumina membranes
Biomedical applications
Electrical stimulations
Nanoporous morphologies
Neural differentiations
PC-12 cells
PC-12 pheochromocytoma cells
Conductive materials
Aluminum oxide
Artificial membrane
Delayed release formulation
Nerve growth factor
Animal
Artificial membrane
Cell adhesion
Chemistry
Delayed release formulation
Drug effects
Electric conductivity
PC12 cell line
Pharmacokinetics
Pharmacology
Rat
Aluminum oxide
Animals
Cell adhesion
Delayed-action preparations
Electric conductivity
Membranes, artificial
Nerve growth factor
PC12 cells
Rats
Permalink
http://hdl.handle.net/11693/36803Published Version (Please cite this version)
http://dx.doi.org/10.1016/j.msec.2016.05.084Collections
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