Short-term dietary restriction in old zebrafish changes cell senescence mechanisms
Author
Arslan-Ergul, A.
Erbaba, B.
Karoglu, E. T.
Halim, D. O.
Adams, M. M.
Date
2016-10Source Title
Neuroscience
Print ISSN
0306-4522
Publisher
Elsevier
Volume
334
Pages
64 - 75
Language
English
Type
ArticleItem Usage Stats
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Show full item recordAbstract
Brain aging is marked by a decline in cognitive abilities and associated with neurodegenerative disorders. Recent studies have shown, neurogenesis continues into adulthood but is known to be decreasing during advancing age and these changes may contribute to cognitive alterations. Advances, which aim to promote better aging are of paramount importance. Dietary restriction (DR) is the only non-genetic intervention that reliably extends life- and health-span. Mechanisms of how and why DR and age affect neurogenesis are not well-understood, and have not been utilized much in the zebrafish, which has become a popular model to study brain aging and neurodegenerative disease due to widely available genetic tools. In this study we used young (8–8.5 months) and old (26–32.5 months) zebrafish as the model to investigate the effects of a short-term DR on actively proliferating cells. We successfully applied a 10-week DR to young and old fish, which resulted in a significant loss of body weight in both groups with no effect on normal age-related changes in body growth. We found that age decreased cell proliferation and increased senescence associated β-galactosidase, as well as shortened telomere lengths. In contrast, DR shortened telomere lengths only in young animals. Neither age nor DR changed the differentiation patterns of glial cells. Our results suggest that the potential effects of DR could be mediated by telomere regulation and whether these are beneficial or negative remains to be determined.
Keywords
AgingBrain
Cell proliferation
Dietary restriction
Telomere
Zebrafish
Beta galactosidase
Beta galactosidase
Adult
Age
Animal cell
Animal experiment
Article
Body growth
Brain region
Brain slice
Cell differentiation
Cell proliferation
Controlled study
Diet restriction
Fish model
Glia cell
Nonhuman
Priority journal
Senescence
Stress
Telomere homeostasis
Weight reduction
Zebra fish
Aging
Animal
Body weight
Brain
Caloric restriction
Cell aging
Cohort analysis
Metabolism
Physiology
Randomization
Telomere
Time factor