Biocatalytic protein membranes fabricated by electrospinning
Ölmez, T. T.
Şeker, U. Ö. Ş.
Reactive and Functional Polymers
26 - 32
Item Usage Stats
In this study, a protein-based catalytic membrane was produced by electrospinning. Membrane activity was characterised in terms of response current for various glucose concentrations. We focused on the preparation of a scaffold by converting a globular protein to other structural forms using catastrophic solvents. A scaffolding protein, bovine serum albumin, and an enzyme, glucose oxidase (GOD), were selected as a model natural carrier matrix and a biologically active agent, respectively. Beta-mercaptoethanol (β-ME) was used to convert the globular protein to an amyloid-like form. A structural stabilising agent, 2,2,2-triflouroethanol (TFE), was used to maintain the final α-helical structure of the amyloid-like protein. The TFE:PBS (phosphate-buffered saline) ratio and various electrospinning parameters were analysed to minimise activity loss. Using this approach, we applied electrospinning to an active enzyme to obtain biocatalytic nanofibrous membranes. After optimising the protein electrospinning process, the activities of the protein nanofibrous membranes were monitored. GOD remained active in the new membrane structure. The highest enzyme activity was observed for the membranes prepared with a 1.5:1 (v:v) TFE:PBS solvent ratio. In that particular case, the immobilized enzyme created a current of 0.7 μA and the apparent activity was 2547 ± 132 U/m2.
Bovine serum albumin
Surface plasmon resonance
Bovine serum albumins
Published Version (Please cite this version)https://doi.org/10.1016/j.reactfunctpolym.2016.03.015
Showing items related by title, author, creator and subject.
Senthamizhan, A.; Balusamy, B.; Uyar, Tamer (Springer Verlag, 2016)The worldwide increase in the number of people suffering from diabetes has been the driving force for the development of glucose sensors. The recent past has devised various approaches to formulate glucose sensors using ...
Systematic hydrolysis of PIM-1 and electrospinning of hydrolyzed PIM-1 ultrafine fibers for an efficient removal of dye from water Satilmis, B.; Budd, P. M.; Uyar, Tamer (Elsevier, 2017-12)In this study, the Polymer of Intrinsic Microporosity (PIM-1) was systematically hydrolyzed in the presence of sodium hydroxide by varying the concentration of base, washing procedure and the time of the reaction. The ...
Fabrication of cellulose acetate/polybenzoxazine cross-linked electrospun nanofibrous membrane for water treatment Ertaş, Yelda; Uyar, Tamer (Elsevier, 2017-12)Herein, polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency was successfully produced via electrospinning and thermal ...