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      Supramolecular GAG-like self-assembled glycopeptide nanofibers Induce chondrogenesis and cartilage regeneration

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      Author(s)
      Yaylaci, U. S.
      Ekiz, M. S.
      Arslan, E.
      Can, N.
      Kilic, E.
      Ozkan, H.
      Orujalipoor, I.
      Ide, S.
      Tekinay, A. B.
      Güler, Mustafa O.
      Date
      2016
      Source Title
      Biomacromolecules
      Print ISSN
      1525-7797
      Publisher
      American Chemical Society
      Volume
      17
      Issue
      2
      Pages
      679 - 689
      Language
      English
      Type
      Article
      Item Usage Stats
      228
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      385
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      Abstract
      Glycosaminoglycans (GAGs) and glycoproteins are vital components of the extracellular matrix, directing cell proliferation, differentiation, and migration and tissue homeostasis. Here, we demonstrate supramolecular GAG-like glycopeptide nanofibers mimicking bioactive functions of natural hyaluronic acid molecules. Self-assembly of the glycopeptide amphiphile molecules enable organization of glucose residues in close proximity on a nanoscale structure forming a supramolecular GAG-like system. Our in vitro culture results indicated that the glycopeptide nanofibers are recognized through CD44 receptors, and promote chondrogenic differentiation of mesenchymal stem cells. We analyzed the bioactivity of GAG-like glycopeptide nanofibers in chondrogenic differentiation and injury models because hyaluronic acid is a major component of articular cartilage. Capacity of glycopeptide nanofibers on in vivo cartilage regeneration was demonstrated in microfracture treated osteochondral defect healing. The glycopeptide nanofibers act as a cell-instructive synthetic counterpart of hyaluronic acid, and they can be used in stem cell-based cartilage regeneration therapies.
      Keywords
      Cell culture
      Cell proliferation
      Cells
      Cytology
      Hyaluronic acid
      Molecules
      Nanofibers
      Organic acids
      Peptides
      Self assembly
      Stem cells
      Supramolecular chemistry
      Tissue
      Tissue homeostasis
      Amphiphile molecules
      Articular cartilages
      Cartilage regeneration
      Chondrogenic differentiation
      Extracellular matrices
      Mesenchymal stem cell
      Nanoscale structure
      Osteochondral defects
      Cartilage
      Hermes antigen
      Cd44 protein, mouse
      Hermes antigen
      Cartilage
      Mesenchymal Stromal Cells
      Molecular Mimicry
      Regeneration
      Scattering, Small Angle
      Tissue Scaffolds
      X-Ray Diffraction
      Permalink
      http://hdl.handle.net/11693/36747
      Published Version (Please cite this version)
      http://dx.doi.org/10.1021/acs.biomac.5b01669
      Collections
      • Institute of Materials Science and Nanotechnology (UNAM) 2098
      • Nanotechnology Research Center (NANOTAM) 1125
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