Angiogenic peptide nanofibers improve wound healing in STZ-induced diabetic rats
Date
2016-06Source Title
ACS Biomaterials Science and Engineering
Print ISSN
2373-9878
Publisher
American Chemical Society
Volume
2
Issue
7
Pages
1180 - 1189
Language
English
Type
ArticleItem Usage Stats
213
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230
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Abstract
Low expressions of angiogenic growth factors delay the healing of diabetic wounds by interfering with the process of blood vessel formation. Heparin mimetic peptide nanofibers can bind to and enhance production and activity of major angiogenic growth factors, including VEGF. In this study, we showed that heparin mimetic peptide nanofibers can serve as angiogenic scaffolds that allow slow release of growth factors and protect them from degradation, providing a new therapeutic way to accelerate healing of diabetic wounds. We treated wounds in STZ-induced diabetic rats with heparin mimetic peptide nanofibers and studied repair of full-thickness diabetic skin wounds. Wound recovery was quantified by analyses of re-epithelialization, granulation tissue formation and blood vessel density, as well as VEGF and inflammatory response measurements. Wound closure and granulation tissue formation were found to be significantly accelerated in heparin mimetic gel treated groups. In addition, blood vessel counts and the expressions of alpha smooth muscle actin and VEGF were significantly higher in bioactive gel treated animals. These results strongly suggest that angiogenic heparin mimetic nanofiber therapy can be used to support the impaired healing process in diabetic wounds.
Keywords
AngiogenesisDiabetic wound healing
Heparin mimetic
Peptide amphiphiles
VEGF expression
Alpha smooth muscle actin
Angiogenesis inhibitor
Growth factor
Heparin derivative
Heparin mimetic peptide
Nanofiber
Streptozocin
Unclassified drug
Vasculotropin
Animal experiment
Animal tissue
Antiangiogenic therapy
Article
Blood vessel
Capillary density
Controlled study
Diabetic wound
Diabetic wound
Epithelization
Gel
Granulation tissue
Inflammation
Male
Nonhuman
Priority journal
Protein degradation
Protein expression
Rat
Skin
Skinfold thickness
Streptozotocin-induced diabetes mellitus
Wound
Wound closure
Wound healing
Permalink
http://hdl.handle.net/11693/36723Published Version (Please cite this version)
https://doi.org/10.1021/acsbiomaterials.6b00238Collections
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