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dc.contributor.authorTansik, G.en_US
dc.contributor.authorKilic, E.en_US
dc.contributor.authorBeter, M.en_US
dc.contributor.authorDemiralp, B.en_US
dc.contributor.authorK.Sendur, G.en_US
dc.contributor.authorCan, N.en_US
dc.contributor.authorOzkan, H.en_US
dc.contributor.authorErgul, E.en_US
dc.contributor.authorGuler, M. O.en_US
dc.contributor.authorTekinay, A. B.en_US
dc.date.accessioned2018-04-12T10:48:25Z
dc.date.available2018-04-12T10:48:25Z
dc.date.issued2016en_US
dc.identifier.issn2047-4830
dc.identifier.urihttp://hdl.handle.net/11693/36684
dc.description.abstractBiomineralization of the extracellular matrix (ECM) plays a crucial role in bone formation. Functional and structural biomimetic native bone ECM components can therefore be used to change the fate of stem cells and induce bone regeneration and mineralization. Glycosaminoglycan (GAG) mimetic peptide nanofibers can interact with several growth factors. These nanostructures are capable of enhancing the osteogenic activity and mineral deposition of osteoblastic cells, which is indicative of their potential application in bone tissue regeneration. In this study, we investigated the potential of GAG-mimetic peptide nanofibers to promote the osteogenic differentiation of rat mesenchymal stem cells (rMSCs) in vitro and enhance the bone regeneration and biomineralization process in vivo in a rabbit tibial bone defect model. Alkaline phosphatase (ALP) activity and Alizarin red staining results suggested that osteogenic differentiation is enhanced when rMSCs are cultured on GAG-mimetic peptide nanofibers. Moreover, osteogenic marker genes were shown to be upregulated in the presence of the peptide nanofiber system. Histological and micro-computed tomography (Micro-CT) observations of regenerated bone defects in rabbit tibia bone also suggested that the injection of a GAG-mimetic nanofiber gel supports cortical bone deposition by enhancing the secretion of an inorganic mineral matrix. The volume of the repaired cortical bone was higher in GAG-PA gel injected animals. The overall results indicate that GAG-mimetic peptide nanofibers can be utilized effectively as a new bioactive platform for bone regeneration. © 2016 The Royal Society of Chemistry.en_US
dc.language.isoEnglishen_US
dc.source.titleBiomaterials Scienceen_US
dc.relation.isversionofhttp://doi.org/10.1039/c6bm00179cen_US
dc.subjectBiomimeticsen_US
dc.subjectBiomineralizationen_US
dc.subjectCell cultureen_US
dc.subjectComputerized tomographyen_US
dc.subjectDefectsen_US
dc.subjectDepositionen_US
dc.subjectNanofibersen_US
dc.subjectPeptidesen_US
dc.subjectPhosphatasesen_US
dc.subjectStem cellsen_US
dc.subjectTissue regenerationen_US
dc.subjectAlkaline phosphatase activityen_US
dc.subjectBiomineralization processen_US
dc.subjectBone tissue regenerationen_US
dc.subjectExtracellular matricesen_US
dc.subjectMesenchymal stem cellen_US
dc.subjectMicro computed tomography (micro-CT)en_US
dc.subjectOsteogenic activityen_US
dc.subjectOsteogenic differentiationen_US
dc.subjectBoneen_US
dc.subjectAlizarin red sen_US
dc.subjectAlkaline phosphataseen_US
dc.subjectGlycosaminoglycan peptideen_US
dc.subjectMolecular scaffolden_US
dc.subjectGlycosaminoglycanen_US
dc.subjectNanofiberen_US
dc.subjectPeptideen_US
dc.subjectAdulten_US
dc.subjectAnimal cellen_US
dc.subjectAnimal experimenten_US
dc.subjectAnimal tissueen_US
dc.subjectBiomineralizationen_US
dc.subjectBone defecten_US
dc.subjectBone developmenten_US
dc.subjectBone regenerationen_US
dc.subjectCell cultureen_US
dc.subjectCell differentiationen_US
dc.subjectControlled studyen_US
dc.subjectCortical boneen_US
dc.subjectEnzyme activityen_US
dc.subjectGelen_US
dc.subjectIn vitro studyen_US
dc.subjectIn vivo studyen_US
dc.subjectMarker geneen_US
dc.subjectMesenchymal stem cellen_US
dc.subjectMicro-computed tomographyen_US
dc.subjectMicroenvironmenten_US
dc.subjectNonhumanen_US
dc.subjectPriority journalen_US
dc.subjectRabbiten_US
dc.subjectRaten_US
dc.subjectStainingen_US
dc.subjectUpregulationen_US
dc.subjectYoung adulten_US
dc.subjectRabbitsen_US
dc.subjectRatsen_US
dc.subjectTissue Scaffoldsen_US
dc.subjectX-Ray Microtomographyen_US
dc.titleA glycosaminoglycan mimetic peptide nanofiber gel as an osteoinductive scaffolden_US
dc.typeArticleen_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.citation.spage1328en_US
dc.citation.epage1339en_US
dc.citation.volumeNumber4en_US
dc.citation.issueNumber9en_US
dc.identifier.doi10.1039/c6bm00179cen_US
dc.publisherRoyal Society of Chemistryen_US


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