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      A portable microfluidic system for rapid measurement of the erythrocyte sedimentation rate

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      Author
      Isiksacan, Z.
      Erel, O.
      Elbuken, C.
      Date
      2016
      Source Title
      Lab on a Chip
      Print ISSN
      1473-0197
      Publisher
      Royal Society of Chemistry
      Volume
      16
      Issue
      24
      Pages
      4682 - 4690
      Language
      English
      Type
      Article
      Item Usage Stats
      118
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      Abstract
      The erythrocyte sedimentation rate (ESR) is a frequently used 30 min or 60 min clinical test for screening of several inflammatory conditions, infections, trauma, and malignant diseases, as well as non-inflammatory conditions including prostate cancer and stroke. Erythrocyte aggregation (EA) is a physiological process where erythrocytes form face-to-face linear structures, called rouleaux, at stasis or low shear rates. In this work, we proposed a method for ESR measurement from EA. We developed a microfluidic opto-electro-mechanical system, using which we experimentally showed a significant correlation (R2 = 0.86) between ESR and EA. The microfluidic system was shown to measure ESR from EA using fingerprick blood in 2 min. 40 μl of whole blood is filled in a disposable polycarbonate cartridge which is illuminated with a near infrared emitting diode. Erythrocytes were disaggregated under the effect of a mechanical shear force using a solenoid pinch valve. Following complete disaggregation, transmitted light through the cartridge was measured using a photodetector for 1.5 min. The intensity level is at its lowest at complete disaggregation and highest at complete aggregation. We calculated ESR from the transmitted signal profile. We also developed another microfluidic cartridge specifically for monitoring the EA process in real-time during ESR measurement. The presented system is suitable for ultrafast, low-cost, and low-sample volume measurement of ESR at the point-of-care.
      Keywords
      Polycarbonate
      Blood sampling
      Calculation
      Erythrocyte aggregation
      Erythrocyte sedimentation rate
      Force
      Human
      Light emitting diode
      Measurement accuracy
      Measurement repeatability
      Microfluidics
      Point of care system
      Portable equipment
      Priority journal
      Shear rate
      Permalink
      http://hdl.handle.net/11693/36679
      Published Version (Please cite this version)
      http://dx.doi.org/10.1039/C6LC01036A
      Collections
      • Institute of Materials Science and Nanotechnology (UNAM) 1775
      • Nanotechnology Research Center (NANOTAM) 1006
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