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      Catalytic supramolecular self-assembled peptide nanostructures for ester hydrolysis

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      Author(s)
      Gulseren, G.
      Khalily, M. A.
      Tekinay, A. B.
      Güler, Mustafa O.
      Date
      2016
      Source Title
      Journal of Materials Chemistry B
      Print ISSN
      2050-7518
      Publisher
      Royal Society of Chemistry
      Volume
      4
      Issue
      26
      Pages
      4605 - 4611
      Language
      English
      Type
      Article
      Item Usage Stats
      287
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      400
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      Abstract
      Essential amino acids in catalytic sites of native enzymes are important in nature inspired catalyst designs. Active sites of enzymes contain the coordinated assembly of multiple amino acids, and catalytic action is generated by the dynamic interactions among multiple residues. However, catalysis studies are limited by the complex and dynamic structure of the enzyme; and it is difficult to exclusively attribute a given function to a specific residue. Minimalistic approaches involving artificial catalytic sites are promising for the investigation of the enzyme function in the absence of non-essential protein components, and self-assembling peptide nanostructures are especially advantageous in this context. Here we demonstrate the design and characterization of an enzyme-mimetic catalytic nanosystem presenting essential residues (Ser, His, Asp). The function of each residue and its combinations on the nanostructures in hydrolysis reaction was studied. The catalytic self-assembled nanostructures were used for efficient ester hydrolysis such as a model substrate (pNPA) and a natural substrate (acetylcholine) highlighting the key role of self-assembly in catalytic domain formation to test the efficiency of the de novo designed catalyst as a catalytic triad model.
      Keywords
      Amino acids
      Biomimetics
      Catalysts
      Enzymes
      Esters
      Hydrolysis
      Peptides
      Self assembly
      Dynamic interaction
      Dynamic structure
      Essential amino acids
      Hydrolysis reaction
      Natural substrates
      Self assembled nanostructures
      Self-assembled peptides
      Self-assembling peptides
      Nanostructures
      Permalink
      http://hdl.handle.net/11693/36666
      Published Version (Please cite this version)
      https://doi.org/10.1039/c6tb00795c
      Collections
      • Institute of Materials Science and Nanotechnology (UNAM) 2258
      • Nanotechnology Research Center (NANOTAM) 1179
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