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dc.contributor.authorSucularli, C.en_US
dc.contributor.authorShehwana, H.en_US
dc.contributor.authorKuscu, C.en_US
dc.contributor.authorDungul, D. C.en_US
dc.contributor.authorOzdag, H.en_US
dc.contributor.authorKonu, O.en_US
dc.date.accessioned2018-04-12T10:40:47Z
dc.date.available2018-04-12T10:40:47Z
dc.date.issued2016-03en_US
dc.identifier.issn1791-2997
dc.identifier.urihttp://hdl.handle.net/11693/36466
dc.description.abstractMechanistic target of rapamycin (mTOR) is a conserved serine/threonine kinase important in cell proliferation, growth and protein translation. Rapamycin, a well-known anti-cancer agent and immunosuppressant drug, inhibits mTOR activity in different taxa including zebrafish. In the present study, the effect of rapamycin exposure on the transcriptome of a zebrafish fibroblast cell line, ZF4, was investigated. Microarray analysis demonstrated that rapamycin treatment modulated a large set of genes with varying functions including protein synthesis, assembly of mitochondrial and proteasomal machinery, cell cycle, metabolism and oxidative phosphorylation in ZF4 cells. A mild however, coordinated reduction in the expression of proteasomal and mitochondrial ribosomal subunits was detected, while the expression of numerous ribosomal subunits increased. Meta-analysis of heterogeneous mouse rapamycin microarray datasets enabled the comparison of zebrafish and mouse pathways modulated by rapamycin, using Kyoto Encyclopedia of Genes and Genomes and Gene Ontology pathway analysis. The analyses demonstrated a high degree of functional conservation between zebrafish and mice in response to rapamycin. In addition, rapamycin treatment resulted in a marked dose-dependent reduction in body size and pigmentation in zebrafish embryos. The present study is the first, to the best of our knowledge, to evaluate the conservation of rapamycin-modulated functional pathways between zebrafish and mice, in addition to the dose-dependent growth curves of zebrafish embryos upon rapamycin exposure.en_US
dc.language.isoEnglishen_US
dc.source.titleMolecular Medicine Reportsen_US
dc.relation.isversionofhttps://doi.org/10.3892/mmr.2016.5059en_US
dc.subjectMeta-analysisen_US
dc.subjectMicroarrayen_US
dc.subjectMouseen_US
dc.subjectmTORen_US
dc.subjectRapamycinen_US
dc.subjectReal-time qPCRen_US
dc.subjectZebrafishen_US
dc.subjectZF4en_US
dc.subjectAromatic levo amino acid decarboxylaseen_US
dc.subjectCytochrome P450en_US
dc.subjectCytochrome P450 26B1en_US
dc.subjectDickkopf 1 proteinen_US
dc.subjectDickkopf 1b proteinen_US
dc.subjectMessenger RNAen_US
dc.subjectPhenylalanine 4 monooxygenaseen_US
dc.subjectProteasomeen_US
dc.subjectProteinen_US
dc.subjectRapamycinen_US
dc.subjectTranscription factoren_US
dc.subjectTranscription factor FOXM1en_US
dc.subjectTranscriptomeen_US
dc.subjectUnclassified drugen_US
dc.subjectWNT inhibitory factor 1en_US
dc.subjectRapamycinen_US
dc.subjectTarget of rapamycin kinaseen_US
dc.subjectZebrafish proteinen_US
dc.subjectAnimal cellen_US
dc.subjectAnimal cell cultureen_US
dc.subjectAnimal experimenten_US
dc.subjectApoptosisen_US
dc.subjectArticleen_US
dc.subjectBody sizeen_US
dc.subjectCell cycleen_US
dc.subjectCell metabolismen_US
dc.subjectCell proliferationen_US
dc.subjectCell viabilityen_US
dc.subjectControlled studyen_US
dc.subjectDNA synthesisen_US
dc.subjectDown regulationen_US
dc.subjectDrug effecten_US
dc.subjectDrug exposureen_US
dc.subjectEmbryoen_US
dc.subjectEmbryo growthen_US
dc.subjectEndoplasmic reticulumen_US
dc.subjectFibroblast cultureen_US
dc.subjectGene expressionen_US
dc.subjectGenetic conservationen_US
dc.subjectMicroarray analysisen_US
dc.subjectMitochondrionen_US
dc.subjectMouseen_US
dc.subjectNonhumanen_US
dc.subjectOxidative phosphorylationen_US
dc.subjectPigmentationen_US
dc.subjectProtein synthesisen_US
dc.subjectRibosome subuniten_US
dc.subjectSteady stateen_US
dc.subjectUpregulationen_US
dc.subjectZebra fishen_US
dc.subjectAnimalen_US
dc.subjectCell lineen_US
dc.subjectComparative studyen_US
dc.subjectDrug effectsen_US
dc.subjectGene expression regulationen_US
dc.subjectMetabolismen_US
dc.subjectSpecies differenceen_US
dc.subjectZebra fishen_US
dc.subjectAnimalsen_US
dc.subjectCell Lineen_US
dc.subjectGene Expression Regulationen_US
dc.subjectMiceen_US
dc.subjectSirolimusen_US
dc.subjectSpecies Specificityen_US
dc.subjectTOR Serine-Threonine Kinasesen_US
dc.subjectZebrafishen_US
dc.subjectZebrafish Proteinsen_US
dc.titleFunctionally conserved effects of rapamycin exposure on zebrafishen_US
dc.typeArticleen_US
dc.departmentDepartment of Molecular Biology and Genetics
dc.citation.spage4421en_US
dc.citation.epage4430en_US
dc.citation.volumeNumber13en_US
dc.citation.issueNumber5en_US
dc.identifier.doi10.3892/mmr.2016.5059en_US
dc.publisherSpandidos Publicationsen_US


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