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      Three-Dimensional Laminin Mimetic Peptide Nanofiber Gels for In Vitro Neural Differentiation

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      Embargo Lift Date: 2018-12-18
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      Author
      Gunay, Gokhan
      Sever, Melike
      Tekinay, Ayse B.
      Güler, Mustafa O.
      Date
      2017
      Source Title
      Biotechnology Journal
      Print ISSN
      1860-6768
      Publisher
      Wiley-VCH Verlag
      Volume
      12
      Issue
      12
      Pages
      1 - 9
      Language
      English
      Type
      Article
      Item Usage Stats
      157
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      193
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      Abstract
      The extracellular matrix (ECM) provides biochemical signals and structural support for cells, and its functional imitation is a fundamental aspect of biomaterial design for regenerative medicine applications. The stimulation of neural differentiation by a laminin protein-derived epitope in two-dimensional (2D) and three-dimensional (3D) environments is investigated. The 3D gel system is found to be superior to its 2D counterpart for the induction of neural differentiation, even in the absence of a crucial biological inducer in nerve growth factor (NGF). In addition, cells cultured in 3D gels exhibits a spherical morphology that is consistent with their form under in vivo conditions. Overall, the present study underlines the impact of bioactivity, dimension, and NGF addition, as well as the cooperative effects thereof, on the neural differentiation of PC-12 cells. These results underline the significance of 3D culture systems in the development of scaffolds that closely replicate in vivo environments for the formation of cellular organoid models in vitro. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
      Keywords
      2D and 3D cell culture
      Extracellular matrix
      Laminin
      Bioactivity
      Biomimetics
      Cell culture
      Cells
      Cytology
      Gels
      Peptides
      3-D cell culture
      Extracellular matrices
      Laminin
      Neural differentiations
      Peptide amphiphiles
      Scaffolds (biology)
      Amphophile
      Beta III tubulin
      Beta tubulin
      Epitope
      Nanofiber
      Nerve growth factor
      Peptidomimetic agent
      Unclassified drug
      Controlled study
      Gene expression
      Hydrogel
      Nerve cell differentiation
      Neurite
      PC12 cell line
      Priority journal
      Protein expression
      Permalink
      http://hdl.handle.net/11693/36417
      Published Version (Please cite this version)
      http://dx.doi.org/10.1002/biot.201700080
      Collections
      • Aysel Sabuncu Brain Research Center (BAM) 201
      • Institute of Materials Science and Nanotechnology (UNAM) 1891
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