Three-Dimensional Laminin Mimetic Peptide Nanofiber Gels for In Vitro Neural Differentiation
Author
Gunay, Gokhan
Sever, Melike
Tekinay, Ayse B.
Güler, Mustafa O.
Date
2017Source Title
Biotechnology Journal
Print ISSN
1860-6768
Publisher
Wiley-VCH Verlag
Volume
12
Issue
12
Pages
1 - 9
Language
English
Type
ArticleItem Usage Stats
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Abstract
The extracellular matrix (ECM) provides biochemical signals and structural support for cells, and its functional imitation is a fundamental aspect of biomaterial design for regenerative medicine applications. The stimulation of neural differentiation by a laminin protein-derived epitope in two-dimensional (2D) and three-dimensional (3D) environments is investigated. The 3D gel system is found to be superior to its 2D counterpart for the induction of neural differentiation, even in the absence of a crucial biological inducer in nerve growth factor (NGF). In addition, cells cultured in 3D gels exhibits a spherical morphology that is consistent with their form under in vivo conditions. Overall, the present study underlines the impact of bioactivity, dimension, and NGF addition, as well as the cooperative effects thereof, on the neural differentiation of PC-12 cells. These results underline the significance of 3D culture systems in the development of scaffolds that closely replicate in vivo environments for the formation of cellular organoid models in vitro. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Keywords
2D and 3D cell cultureExtracellular matrix
Laminin
Bioactivity
Biomimetics
Cell culture
Cells
Cytology
Gels
Peptides
3-D cell culture
Extracellular matrices
Laminin
Neural differentiations
Peptide amphiphiles
Scaffolds (biology)
Amphophile
Beta III tubulin
Beta tubulin
Epitope
Nanofiber
Nerve growth factor
Peptidomimetic agent
Unclassified drug
Controlled study
Gene expression
Hydrogel
Nerve cell differentiation
Neurite
PC12 cell line
Priority journal
Protein expression