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dc.contributor.advisorTekinay, Ayşe Begümen_US
dc.contributor.authorOrhan, Zeynepen_US
dc.date.accessioned2016-11-21T08:51:46Z
dc.date.available2016-11-21T08:51:46Z
dc.date.copyright2016-11
dc.date.issued2016-11
dc.date.submitted2016-11-14
dc.identifier.urihttp://hdl.handle.net/11693/32526
dc.descriptionCataloged from PDF version of article.en_US
dc.descriptionThesis (M.S.): Bilkent University, Department of Neuroscience, İhsan Doğramacı Bilkent University, 2016.en_US
dc.descriptionIncludes bibliographical references (leaves 83-95).en_US
dc.description.abstractPromotion of neurite outgrowth is an important limiting step for the regeneration of nerve injury and depends strongly on the local expression of nerve growth factor (NGF). Rational design of bioactive materials is a promising approach for the development of novel therapeutic methods for nerve regeneration, and biomaterials capable of presenting NGF to nerve cells are especially suitable for this purpose. This thesis describes development of nanofibrous peptide amphiphile (PA) nanofibers capable of promoting neurite outgrowth by displaying high density binding epitopes for NGF. The high-affinity NGF-binding sequence was identified by phage display and combined with a beta-sheet forming motif to produce a self-assembling PA molecule. Our results revealed that the bioactive nanofiber had higher affinity for NGF compared to control nanofiber and in vitro studies showed that the NGF binding peptide amphiphile nanofibers (NGFB-PA nanofiber) significantly promote the neurite outgrowth of PC-12 cells. In addition, the nanofibers induced differentiation of PC-12 cells into neuron-like cells by enhancing NGF/high-activity NGF receptor (TrkA) interactions and activating MAPK pathway elements. The first time with this study a seven amino acid phage display peptide library was utilized for high affinity epitope screening for NGF, the NGF binding sequence was incorporated into peptide amphiphile structure, and the effect of NGF binding material on differentiation pathway of NGF was analyzed. This material will pave the way for development of new therapeutic agents for nervous system injuries.en_US
dc.description.statementofresponsibilityby Zeynep Orhan.en_US
dc.format.extentxv, 97 leaves : illustrations (some color), graphics.en_US
dc.language.isoEnglishen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNerve growth factoren_US
dc.subjectEpitope screeningen_US
dc.subjectPhage displayen_US
dc.subjectNeural differentiationen_US
dc.subjectPC-12 cellsen_US
dc.subjectPeptide amphiphilesen_US
dc.titleDesign and application of nerve growth factor-β binding peptide nanofibers for neural regenerationen_US
dc.title.alternativeNGF'de bağlanan peptit nanofiberlerin dizaynı ve nöral rejenerasyon çalışmalarında uygulanmasıen_US
dc.typeThesisen_US
dc.departmentGraduate Program in Neuroscienceen_US
dc.publisherBilkent Universityen_US
dc.description.degreeM.S.en_US
dc.identifier.itemidB154648
dc.embargo.release2018-11-14


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