dc.contributor.advisor | Yavuzer, Uğur | |
dc.contributor.author | Öncel, Dilhan | |
dc.date.accessioned | 2016-07-01T11:06:09Z | |
dc.date.available | 2016-07-01T11:06:09Z | |
dc.date.issued | 2001 | |
dc.identifier.uri | http://hdl.handle.net/11693/29823 | |
dc.description | Cataloged from PDF version of article. | en_US |
dc.description.abstract | The nuclear matrix protein C1D is an activator of DNA-dependent protein kinase (DNAPK),
which is involved in non-homologous end joining and V(D)J recombination. Moreover, the
nuclear matrix proteins C1D was shown to be phosphorylated in vitro by DNA-PK and its mRNA
and protein levels have been demonstrated to be induced upon γ-irradiation. The available
information suggests that C1D may play a role in DNA DSB-response pathways by targeting DNAPKcs
to nuclear matrix and matrix-associated DNA where DNA-PK may regulate several cellular
processes. In an attempt to identify the biological function of C1D, the yeast two-hybrid system
was employed and C1D was found to interact with a partial clone of TAFI 48, which is a subunit of
the promoter selectivity factor SL1 required for the accurate initiation of transcription from the
human ribosomal promoter. The possibility of an interaction between C1D and TAFI 48 was
potentially important as DNA-PK was found to be capable of repressing Polymerase I transcription
in vitro.
In this study, it was shown that C1D and TAFI 48 interact specifically in yeast, in vitro and
in mammalian cells. It ıs belıeved that this interaction could have potential roles in regulating the
repression of transcription from rDNA by DNA-PK in response to DNA double-strand breaks. | en_US |
dc.description.statementofresponsibility | Öncel, Dilhan | en_US |
dc.format.extent | 88 leaves, illustrations | en_US |
dc.language.iso | English | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | C1D | en_US |
dc.subject | DNA-PK | en_US |
dc.subject | DNA Double-strand break | en_US |
dc.subject | TAFI 48 | en_US |
dc.subject.lcc | QH603.N83 O53 2001 | en_US |
dc.subject.lcsh | Nuclear matrix. | en_US |
dc.title | Role of the nuclear matrix protein C1D in regulation of transcription | en_US |
dc.type | Thesis | en_US |
dc.department | Department of Moleculer Biology | en_US |
dc.publisher | Bilkent University | en_US |
dc.description.degree | M.S. | en_US |
dc.identifier.itemid | BILKUTUPB059760 | |