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dc.contributor.advisorYavuzer, Uğur
dc.contributor.authorÖncel, Dilhan
dc.date.accessioned2016-07-01T11:06:09Z
dc.date.available2016-07-01T11:06:09Z
dc.date.issued2001
dc.identifier.urihttp://hdl.handle.net/11693/29823
dc.descriptionCataloged from PDF version of article.en_US
dc.description.abstractThe nuclear matrix protein C1D is an activator of DNA-dependent protein kinase (DNAPK), which is involved in non-homologous end joining and V(D)J recombination. Moreover, the nuclear matrix proteins C1D was shown to be phosphorylated in vitro by DNA-PK and its mRNA and protein levels have been demonstrated to be induced upon γ-irradiation. The available information suggests that C1D may play a role in DNA DSB-response pathways by targeting DNAPKcs to nuclear matrix and matrix-associated DNA where DNA-PK may regulate several cellular processes. In an attempt to identify the biological function of C1D, the yeast two-hybrid system was employed and C1D was found to interact with a partial clone of TAFI 48, which is a subunit of the promoter selectivity factor SL1 required for the accurate initiation of transcription from the human ribosomal promoter. The possibility of an interaction between C1D and TAFI 48 was potentially important as DNA-PK was found to be capable of repressing Polymerase I transcription in vitro. In this study, it was shown that C1D and TAFI 48 interact specifically in yeast, in vitro and in mammalian cells. It ıs belıeved that this interaction could have potential roles in regulating the repression of transcription from rDNA by DNA-PK in response to DNA double-strand breaks.en_US
dc.description.statementofresponsibilityÖncel, Dilhanen_US
dc.format.extent88 leaves, illustrationsen_US
dc.language.isoEnglishen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectC1Den_US
dc.subjectDNA-PKen_US
dc.subjectDNA Double-strand breaken_US
dc.subjectTAFI 48en_US
dc.subject.lccQH603.N83 O53 2001en_US
dc.subject.lcshNuclear matrix.en_US
dc.titleRole of the nuclear matrix protein C1D in regulation of transcriptionen_US
dc.typeThesisen_US
dc.departmentDepartment of Moleculer Biologyen_US
dc.publisherBilkent Universityen_US
dc.description.degreeM.S.en_US
dc.identifier.itemidBILKUTUPB059760


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