Cloning and expression profile of FLT3 gene during rat liver regeneration
Author
Aydın, Iraz Toprak
Advisor
Akçalı, K. Can
Date
2005Publisher
Bilkent University
Language
English
Type
ThesisItem Usage Stats
96
views
views
30
downloads
downloads
Abstract
Liver has a unique capacity to regenerate itself upon exposure to viral infections,
toxic reactions and cancer formation which result in the loss of hepatocyte. Liver
regeneration is a complex phenomenon in which several factors participate during
its onset. Cellular proliferation is one of the important component of this process
and the factors regulate this proliferation has a vital role. FLT3, a well-known
hematopoietic stem cell and hepatic lineage surface marker, takes place in proliferative
events of hematopoietic stem cells. However, its contribution to liver
regeneration is not known. Therefore, in this study, we aimed to examine the role
of FLT3 during liver regeneration. First we cloned a partial cDNA of rat homolog
of FLT3 from thymus and examined the tissue specific expressions both in mRNA
and protein level. After performing hepatectomy in rats to induce liver regeneration,
expression profile of FLT3 in both mRNA and protein level and its cellular
localization were also investigated during the different stages of liver regeneration
models. Our data showed that FLT3 is expressed in most of the rat tissues and
in liver regeneration. However, its intracellular localization is changed during the
late stages of liver regeneration. Therefore, our results suggest a mechanism in
which FLT3 receptor is activated in the late stages of liver regeneration.