dc.contributor.advisor | Yağcı, Tamer | |
dc.contributor.author | Çelikkaya, Hilal | |
dc.date.accessioned | 2016-07-01T11:00:46Z | |
dc.date.available | 2016-07-01T11:00:46Z | |
dc.date.issued | 2004 | |
dc.identifier.uri | http://hdl.handle.net/11693/29517 | |
dc.description | Cataloged from PDF version of article. | en_US |
dc.description.abstract | Hybridoma technology is a highly specific technique utilized for the production of
monoclonal antibodies. In cancer research, monoclonal antibodies are used as tumor
markers for diagnosis of malignant tissue versus benign or normal, for differential
diagnosis of tumor type, for pathological grading of tumor biopsy specimens, for
detection of the antigens that are up- or down-regulated in tumor cells or in sera of
cancer patients, and for detection of primary or metastatic lesions. Additionally,
monoclonal antibodies are employed in predicting the course of the disease, in
diagnostic approaches for carrying imaging reagents to tumors, and in therapy for
targeting cytotoxic reagents to and triggering or blocking cell surface molecules. This
study involved generation of 6D5 and 9C11 monoclonal antibodies against apoptosis
induced hepatocellular carcinoma cell line HuH-7, followed by characterization
experiments. 6D5 antibody recognized 5 different epitopes in a panel of 28 cell lines in
Western blotting experiments. In immunohistochemistry studies, 6D5 demonstrated
positive staining in cirrhotic and cancerous cells of liver cancer tissue samples. On the
other hand, 9C11 antibody recognized a single band in the same panel of 28 cell lines
but it was not immunoreactive in immunoperixodase studies of liver cancer tissue
samples, under our experimental conditions. | en_US |
dc.description.statementofresponsibility | Çelikkaya, Hilal | en_US |
dc.format.extent | xii, 75 leaves, illustrations | en_US |
dc.language.iso | English | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject.lcc | QR185.8.H93 C45 2004 | en_US |
dc.subject.lcsh | Hybridomas. | en_US |
dc.title | Investigation of novel tumor markers based on hybridoma technology | en_US |
dc.type | Thesis | en_US |
dc.department | Department of Moleculer Biology | en_US |
dc.publisher | Bilkent University | en_US |
dc.description.degree | M.S. | en_US |
dc.identifier.itemid | BILKUTUPB021094 | |