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dc.contributor.advisorGüler, Mustafa Özgüren_US
dc.contributor.authorÇalışkan, Özüm Şehnaz.en_US
dc.date.accessioned2016-06-07T08:04:39Z
dc.date.available2016-06-07T08:04:39Z
dc.date.copyright2016-05
dc.date.issued2016-05
dc.date.submitted2016-06-01
dc.identifier.urihttp://hdl.handle.net/11693/29140
dc.descriptionCataloged from PDF version of article.en_US
dc.descriptionIncludes bibliographical references (leaves 115-126).en_US
dc.descriptionThesis (M.S.): Bilkent University, Department of Materials Science and Nanotechnology, İhsan Doğramacı Bilkent University, 2016.en_US
dc.description.abstractNatural extracellular matrix (ECM) is rich in glycopeptides and glycosaminoglycans, which function in controlling cellular processes. In this thesis, glycopeptide molecules that mimic natural glycopeptides and glycosaminoglycans were designed and synthesized and it was demonstrated that they induce directed differentiation of mesenchymal stem cells into chondrogenic and adipogenic lineages. In the first part of the study, hyaluronic acid (HA)-mimicking glycopeptide amphiphile molecules were synthesized to induce chondrogenic differentiation of mesenchymal stem cells (MSC). HA is the most abundant glycosaminoglycan (GAG) found in hyaline cartilage ECM. Peptide amphiphiles were synthesized by solid phase peptide synthesis method and used to form self-assembled bioactive glycopeptide nanofibers which mimic fibrous morphology of the ECM. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and circular dichroism (CD) were used for morphology and secondary structure analyses of the obtained nanofibers. It was demonstrated that glycopeptide amphiphiles create fibrous structure formed by nanofibers. Morphological changes, GAG production (Safranin-O staining and DMMB analysis), and chondrogenic gene marker expressions (qRT-PCR) of MSCs cultured on HA-mimetic nanofibers were analyzed. It was shown that HA-mimetic glycopeptide nanofibers induce early differentiation of MSCs into hyaline like chondrocytes. In the second part of the study, it was demonstrated that minor changes on glycopeptide backbone can create specific glycopeptides which induce differentiation of MSCs into brown adipocytes. Brown fat adipocytes do not store chemical energy as fat but dissipates it as heat and so they have emerged as promising anti-obesity agents. Lipid droplet accumulation (Oil Red-O staining) and adipogenic gene marker expression analyses (qRT-PCR) showed that the new glycopeptide nanofiber scaffold is a specific inducer of differentiation of MSCs into brown fat adipocytes.en_US
dc.description.statementofresponsibilityby Özüm Şehnaz Çalışkan.en_US
dc.format.extentxxi, 126 pages : illustrations (some colour), charts.en_US
dc.language.isoEnglishen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPeptide amphiphileen_US
dc.subjectPeptide nanofiberen_US
dc.subjectGlycosaminoglycanen_US
dc.subjectGlycopeptideen_US
dc.subjectMesenchymal stem cellen_US
dc.subjectExtracellular matrixen_US
dc.subjectHyaluronic aciden_US
dc.subjectMesenchymal stem cell differentiationen_US
dc.subjectCartilage tissueen_US
dc.subjectTissue regenerationen_US
dc.subjectBrown fat tissueen_US
dc.subjectAdipogenesisen_US
dc.titleBioactive glycopeptide nanofibers for tissue regeneration applicationsen_US
dc.title.alternativeBiyoaktif glikopeptit nanofiberlerin doku rejenerasyonu uygulamalarıen_US
dc.typeThesisen_US
dc.departmentGraduate Program in Materials Science and Nanotechnologyen_US
dc.publisherBilkent Universityen_US
dc.description.degreeM.S.en_US
dc.identifier.itemidB153385
dc.embargo.release2018-05-27


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