dc.contributor.advisor | Tekinay, Ayşe Begüm | |
dc.contributor.author | Yaylacı, Seher | |
dc.date.accessioned | 2016-05-05T07:36:52Z | |
dc.date.available | 2016-05-05T07:36:52Z | |
dc.date.copyright | 2015-09 | |
dc.date.issued | 2015-09 | |
dc.date.submitted | 2015-09-14 | |
dc.identifier.uri | http://hdl.handle.net/11693/29074 | |
dc.description | Cataloged from PDF version of thesis. | en_US |
dc.description | Includes bibliographical references (leaves 127-140). | en_US |
dc.description | Thesis (Ph. D.): Bilkent University, Materials Science and Nanotechnology Program, İhsan Doğramacı Bilkent University, 2015. | en_US |
dc.description.abstract | Articular cartilage is a tissue that is continuously exposed to cyclical compressive
stresses, but exhibits no capacity for self-healing following trauma. Cartilage has a
dense extracellular matrix that is sparsely populated with cells, and the whole tissue
lacks blood and lymphatic vessels, which complicates the cell infiltration response
that ordinarily occurs during inflammation. In addition, the only cell type capable of
synthesizing new cartilage matrix lies deeper in the tissue, near the bone boundary,
and due to the dense extracellular matrix, chondrocytes cannot migrate to the defect
site following injury. Consequently, cartilage tissue cannot effectively respond to
treatment options. Treatment options exist for the short-term reduction of pain in
smaller defects, but larger injuries necessitate tissue donation, and there is a severe
shortage of articular cartilage that can be donated for autografting.
Microfracture and autologous chondrocyte implantation are the current treatment
options that use cellular therapy for the repair of cartilage. However, the cartilage tissue that forms in the course of these treatments is not the functional hyaline
cartilage, but rather fibrous cartilage, which is mechanically weaker and degenerates
over time. Tissue engineering studies using biodegradable scaffolds and autologous
cells are gaining importance as effective long-term treatment options for the postinjury
production of hyaline cartilage. Such scaffold systems are designed to be
biodegradable and bioactive, which allows them to induce new tissue formation in
shorter periods of time.
In this dissertation, peptide nanofibers mimicking glycosaminoglycan molecules,
which are important constituents of cartilage extracellular matrix, are designed and
the effectiveness of these materials in terms of chondrocyte differentation are tested
under in vitro conditions. As a follow-up study to in vitro experiments, the capacity
of bioactive peptide nanofibers to support cartilage regeneration is evaluated in the
rabbit osteochondral defect model. Structural and mechanical properties of newly
deposited cartilage are highly dependent on the quality and quantity of its
extracellular matrix, which also has a major impact on the integration of replacement
cartilage into the surrounding healthy tissue. Signals provided by bioactive peptide
nanofibers to cells at the defect site can strongly alter the quality of the newly
synthesized extracellular matrix. Consequently, we designed glycosaminoglycanmimetic
peptide nanofibers that closely imitate the structure of the native cartilage
extracellular matrix and demonstrated that these nanofiber networks are able to
induce the synthesis of collagen II and aggrecan molecules, which are the main
constituents of cartilage tissue, during chondrogenic differentiation. | en_US |
dc.description.statementofresponsibility | by Seher Yaylacı. | en_US |
dc.format.extent | xvii, 141 leaves : illustrations (some colour), charts, graphics. | en_US |
dc.language.iso | English | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Cartilage regeneration | en_US |
dc.subject | Mesenchymal stem cells | en_US |
dc.subject | Biomaterials | en_US |
dc.subject | Peptide amphiphile nanofibers | en_US |
dc.subject | Glycosaminoglycans | en_US |
dc.title | Development and characterization of peptide nanofibers for cartilage regeneration | en_US |
dc.title.alternative | Kıkırdak rejenerasyonu için peptit nanofiberlerin geliştirilmesi ve karakterizasyonu | en_US |
dc.type | Thesis | en_US |
dc.department | Graduate Program in Materials Science and Nanotechnology | en_US |
dc.publisher | Bilkent University | en_US |
dc.description.degree | Ph.D. | en_US |
dc.identifier.itemid | B151258 | |
dc.embargo.release | 2017-09-09 | |