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      • Department of Mechanical Engineering
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      Spiral microfluidics device for continuous flow PCR

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      Author
      Salemmilani, Reza
      Çetin, Barbaros
      Date
      2013
      Source Title
      Proceedings of the ASME 2017 Summer Heat Transfer Conference (HT2017)
      Publisher
      ASME
      Volume
      2
      Language
      English
      Type
      Conference Paper
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      Abstract
      Polymerase-chain-Reaction (PCR) is a thermal cycling (repeated heating and cooling of PCR solution) process for DNA amplification. PCR is the key ingredient in many biomedical applications. One key feature for the success of the PCR is to control the temperature of the solution precisely at the desired temperature levels required for the PCR in a cyclic manner. Microfluidics offers a great advantage over conventional techniques since minute amounts of PCR solution can be heated and cooled with a high rate in a controlled manner. In this study, a microfluidic platform has been proposed for continuous-flow PCR. The microfluidic device consists of a spiral channel on a glass wafer with integrated chromium microheaters. Sub-micron thick microheaters are deposited beneath the micro-channels to facilitate localized heating. The microfluidic device is modeled using COMSOL MultiphysicsR . The fabrication procedure of the device is also discussed and future research directions are addressed. With its compact design, the proposed system can easily be coupled with an integrated microfluidic device to be used in biomedical applications. Copyright © 2013 by ASME.
      Keywords
      Biomedical applications
      Conventional techniques
      Fabrication procedure
      Future research directions
      Integrated microfluidic devices
      Micro-fluidic devices
      Microfluidic platforms
      Microfluidics devices
      Fluidic devices
      Fuel cells
      Heat transfer
      Heating equipment
      Mass transfer
      Medical applications
      Microelectromechanical devices
      Microfluidics
      Sustainable development
      Polymerase chain reaction
      Permalink
      http://hdl.handle.net/11693/27935
      Published Version (Please cite this version)
      http://dx.doi.org/10.1115/HT2013-17305
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      • Department of Mechanical Engineering 272
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