Spiral microfluidics device for continuous flow PCR
Proceedings of the ASME 2017 Summer Heat Transfer Conference (HT2017)
Item Usage Stats
MetadataShow full item record
Polymerase-chain-Reaction (PCR) is a thermal cycling (repeated heating and cooling of PCR solution) process for DNA amplification. PCR is the key ingredient in many biomedical applications. One key feature for the success of the PCR is to control the temperature of the solution precisely at the desired temperature levels required for the PCR in a cyclic manner. Microfluidics offers a great advantage over conventional techniques since minute amounts of PCR solution can be heated and cooled with a high rate in a controlled manner. In this study, a microfluidic platform has been proposed for continuous-flow PCR. The microfluidic device consists of a spiral channel on a glass wafer with integrated chromium microheaters. Sub-micron thick microheaters are deposited beneath the micro-channels to facilitate localized heating. The microfluidic device is modeled using COMSOL MultiphysicsR . The fabrication procedure of the device is also discussed and future research directions are addressed. With its compact design, the proposed system can easily be coupled with an integrated microfluidic device to be used in biomedical applications. Copyright © 2013 by ASME.
Future research directions
Integrated microfluidic devices
Polymerase chain reaction
Published Version (Please cite this version)http://dx.doi.org/10.1115/HT2013-17305
Showing items related by title, author, creator and subject.
Çaǧatay, E.; Özer, M. B.; Çetin, Barbaros (Chemical and Biological Microsystems Society, 2016)This study focuses on understanding of the sensitivities of the acoustophoretic process on uncertainties/errors in the geometric properties of the chip material and the piezoelectric actuators. The sensitivity of the ...
Guler, M. T.; Bilican, I.; Agan, S.; Elbuken, C. (Institute of Physics Publishing, 2015)In this paper, we present a very simple method to fabricate three-dimensional (3D) microelectrodes integrated with microfluidic devices. We form the electrodes by etching a microwire placed across a microchannel. For precise ...
Isiksacan, Z.; Guler, M. T.; Aydogdu, B.; Bilican, I.; Elbuken, C. (Institute of Physics Publishing, 2016)The conventional fabrication methods for microfluidic devices require cleanroom processes that are costly and time-consuming. We present a novel, facile, and low-cost method for rapid fabrication of polydimethylsiloxane ...