Spiral microfluidics device for continuous flow PCR
ASME 2013 Heat Transfer Summer Conf. Collocated with the ASME 2013 7th Int. Conf. on Energy Sustainability and the ASME 2013 11th Int. Conf. on Fuel Cell Science, Engineering and Technology, HT 2013
MetadataShow full item record
Please cite this item using this persistent URLhttp://hdl.handle.net/11693/27935
Polymerase-chain-Reaction (PCR) is a thermal cycling (repeated heating and cooling of PCR solution) process for DNA amplification. PCR is the key ingredient in many biomedical applications. One key feature for the success of the PCR is to control the temperature of the solution precisely at the desired temperature levels required for the PCR in a cyclic manner. Microfluidics offers a great advantage over conventional techniques since minute amounts of PCR solution can be heated and cooled with a high rate in a controlled manner. In this study, a microfluidic platform has been proposed for continuous-flow PCR. The microfluidic device consists of a spiral channel on a glass wafer with integrated chromium microheaters. Sub-micron thick microheaters are deposited beneath the micro-channels to facilitate localized heating. The microfluidic device is modeled using COMSOL MultiphysicsR . The fabrication procedure of the device is also discussed and future research directions are addressed. With its compact design, the proposed system can easily be coupled with an integrated microfluidic device to be used in biomedical applications. Copyright © 2013 by ASME.
- Conference Paper 2294
Showing items related by title, author, creator and subject.
Çaǧatay E.; Özer M.B.; Çetin B. (Chemical and Biological Microsystems Society, 2016)This study focuses on understanding of the sensitivities of the acoustophoretic process on uncertainties/errors in the geometric properties of the chip material and the piezoelectric actuators. The sensitivity of the ...
Yildirim, A.; Yunusa, M.; Ozturk F.E.; Kanik, M.; Bayindir, M. (Wiley-VCH Verlag, 2014)This article introduces surface textured polymer fibers as a new platform for the fabrication of affordable microfluidic devices. Fibers are produced tens of meters-long at a time and comprise 20 continuous and ordered ...
Sterilization of PMMA microfluidic chips by various techniques and investigation of material characteristics Yavuz C.; Oliaei S.N.B.; Cetin B.; Yesil-Celiktas O. (Elsevier, 2016)The sterilization of microfluidic chips is a vital step of the fabrication process prior to the customer use in biomedical applications. The aim of this study was to analyze the influence of different sterilization techniques ...