Dual functionality of conjugated polymer nanoparticles as an anticancer drug carrier and a fluorescent probe for cell imaging
Author
Gezici, Ö.
Durmaz, I.
Güven, E. B.
Ünal, Ö.
Özgün, A.
Cetin Atalay, R.
Tuncel, D.
Date
2014Source Title
RSC Advances
Print ISSN
2046-2069
Publisher
Royal Society of Chemistry
Volume
4
Issue
3
Pages
1302 - 1309
Language
English
Type
ArticleItem Usage Stats
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Abstract
Multifunctional nanoparticles based on a green emitting, hydrophobic conjugated polymer, poly[(9,9-bis{propeny}fluorenyl-2,7-diyl)-co-(1,4-benzo-{2,1,3}-thiodiazole)] (PPFBT), that acts both as a fluorescent reporter and a matrix to accommodate an anti-cancer compound, camptothecin (CPT), were prepared, characterized and their potential as a fluorescent probe for cell imaging and as a drug delivery vehicle were evaluated via in vitro cell assays. The cell viability of human hepatocellular carcinoma cell line (Huh7) was investigated in the absence and presence of CPT with sulforhodamine B (SRB) and real-time cell electronic sensing (RT-CES) cytotoxicity assays.
Keywords
Camptothecin (CPT)Cytotoxicity assays
Drug delivery vehicles
Fluorescent reporter
Hepatocellular carcinoma cell
Multi-functional nanoparticles
Polymer nanoparticles
Real-time cell electronic sensing
Drug delivery
Cell culture