Induction of triacylglycerol production in Chlamydomonas reinhardtii: comparative analysis of different element regimes
379 - 387
Item Usage Stats
In this study, impacts of different element absence (nitrogen, sulfur, phosphorus and magnesium) and supplementation (nitrogen and zinc) on element uptake and triacylglycerol production was followed in wild type Chlamydomonas reinhardtii CC-124 strain. Macro- and microelement composition of C. reinhardtii greatly differed under element regimes studied. In particular, heavy metal quotas of the microalgae increased strikingly under zinc supplementation. Growth was suppressed, cell biovolume, carbohydrate, total neutral lipid and triacylglycerol levels increased when microalgae were incubated under these element regimes. Most of the intracellular space was occupied by lipid bodies under all nutrient starvations, as observed by confocal microscopy and transmission electron micrographs. Results suggest that sulfur, magnesium and phosphorus deprivations are superior to nitrogen deprivation for the induction triacylglycerol production in C. reinhardtii. On the other hand, FAME profiles of the nitrogen, sulfur and phosphorus deprived cells were found to meet the requirements of international standards for biodiesel.
Transmission electron micrograph
Transmission electron microscopy
Gas Chromatography-Mass Spectrometry
Microscopy, Electron, Transmission
Spectroscopy, Fourier Transform Infrared
Published Version (Please cite this version)http://dx.doi.org/10.1016/j.biortech.2013.12.093
Showing items related by title, author, creator and subject.
Differential effects of nitrogen and sulfur deprivation on growth and biodiesel feedstock production of Chlamydomonas reinhardtii Cakmak, T.; Angun P.; Demiray, Y.E.; Ozkan, A.D.; Elibol, Z.; Tekinay, T. (2012)Biodiesel production from microalgae is a promising approach for energy production; however, high cost of its process limits the use of microalgal biodiesel. Increasing the levels of triacylglycerol (TAG) levels, which is ...
Nitrogen and sulfur deprivation differentiate lipid accumulation targets of chlamydomonas reinhardtii Cakmak, T.; Angun P.; Ozkan, A.D.; Cakmak, Z.; Olmez, T.T.; Tekinay, T. (2012)Nitrogen (N) and sulfur (S) have inter-related and distinct impacts on microalgal metabolism; with N starvation having previously been reported to induce elevated levels of the biodiesel feedstock material triacylglycerol ...
Akturk, M.; Altinova, A.; Mert I.; Dincel, A.; Sargin, A.; Buyukkagnici, U.; Arslan, M.; Danisman, N. (2010)As shown in the previous studies, asymmetric dimethylarginine (ADMA) is related to endothelial dysfunction, whereas high-sensitive C-reactive protein (hCRP) is the marker of inflammation. In our study, we investigated ADMA, ...