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      Induction of triacylglycerol production in Chlamydomonas reinhardtii: comparative analysis of different element regimes

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      Author
      Çakmak, Z. E.
      Ölmez, T. T.
      Çakmak, T.
      Menemen, Y.
      Tekinay, T.
      Date
      2014
      Source Title
      Bioresource Technology
      Print ISSN
      0960-8524
      Publisher
      Elsevier
      Volume
      155
      Pages
      379 - 387
      Language
      English
      Type
      Article
      Item Usage Stats
      138
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      138
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      Abstract
      In this study, impacts of different element absence (nitrogen, sulfur, phosphorus and magnesium) and supplementation (nitrogen and zinc) on element uptake and triacylglycerol production was followed in wild type Chlamydomonas reinhardtii CC-124 strain. Macro- and microelement composition of C. reinhardtii greatly differed under element regimes studied. In particular, heavy metal quotas of the microalgae increased strikingly under zinc supplementation. Growth was suppressed, cell biovolume, carbohydrate, total neutral lipid and triacylglycerol levels increased when microalgae were incubated under these element regimes. Most of the intracellular space was occupied by lipid bodies under all nutrient starvations, as observed by confocal microscopy and transmission electron micrographs. Results suggest that sulfur, magnesium and phosphorus deprivations are superior to nitrogen deprivation for the induction triacylglycerol production in C. reinhardtii. On the other hand, FAME profiles of the nitrogen, sulfur and phosphorus deprived cells were found to meet the requirements of international standards for biodiesel.
      Keywords
      Chlamydomonas reinhardtii
      Ionome
      Neutral lipid
      Nutrient regime
      Triacylglycerol
      Chlamydomonas reinhardtii
      International standards
      Ionome
      Microelement composition
      Neutral lipid
      Phosphorus deprivations
      Transmission electron micrograph
      Triacylglycerols
      Algae
      Heavy metals
      Magnesium
      Microorganisms
      Nitrogen
      Nutrients
      Phosphorus
      Sulfur
      Zinc
      Glycerol
      Carbohydrate
      Copper
      Iron
      Magnesium
      Molybdenum
      Nitrogen
      Phosphorus
      Potassium
      Sulfur
      Triacylglycerol
      Zinc
      Biofuel
      Nile red
      Oxazine derivative
      Triacylglycerol
      Alcohol
      Biochemical composition
      Biotechnology
      Comparative study
      Food supplementation
      Green alga
      Growth rate
      Lipid
      Nutrient uptake
      Nutritional status
      Article
      Bacterial growth
      Cell density
      Cell growth
      Cell structure
      Chlamydomonas reinhardtii
      Confocal microscopy
      Controlled study
      Dry weight
      Growth rate
      Intracellular space
      Lipid storage
      Lipogenesis
      Microalga
      Nonhuman
      Nutrient limitation
      Priority journal
      Starvation
      Wild type
      Bioreactor
      Biosynthesis
      Chlamydomonas reinhardtii
      Comparative study
      Deficiency
      Infrared spectroscopy
      Mass fragmentography
      Mass spectrometry
      Metabolism
      Transmission electron microscopy
      Biofuels
      Bioreactors
      Chlamydomonas reinhardtii
      Gas Chromatography-Mass Spectrometry
      Magnesium
      Mass Spectrometry
      Microscopy, Confocal
      Microscopy, Electron, Transmission
      Nitrogen
      Oxazines
      Phosphorus
      Spectroscopy, Fourier Transform Infrared
      Sulfur
      Triglycerides
      Permalink
      http://hdl.handle.net/11693/26323
      Published Version (Please cite this version)
      http://dx.doi.org/10.1016/j.biortech.2013.12.093
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