Spectroscopic evaluation of DNA–borate Interactions
Date
2015Source Title
Biological Trace Element Research
Print ISSN
0163-4984
Publisher
Humana Press Inc.
Volume
168
Issue
2
Pages
508 - 515
Language
English
Type
ArticleItem Usage Stats
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Abstract
We describe the binding characteristics of two natural borates (colemanite and ulexite) to calf thymus DNA by UV–vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and a competitive DNA binding assay. Our results suggest that colemanite and ulexite interact with calf thymus DNA under a non-intercalative mode of binding and do not alter the secondary structure of the DNA helix. The FT-IR spectroscopy results indicate that the two borates might interact with DNA through sugar-phosphate backbone binding. © 2015, Springer Science+Business Media New York.
Keywords
BoronCalf thymus DNA
Deoxyribose sugar
DNA binding
Spectroscopy
Boric acid
Colemanite
DNA
Sugar phosphate
Ulexite
Unclassified drug
Chemical structure
Circular dichroism
Competitive binding assay
Controlled study
DNA helix
DNA structure
Infrared spectroscopy
Interactions with DNA
Ultraviolet spectroscopy