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      • Department of Molecular Biology and Genetics
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      Characterization of a novel zebrafish (Danio rerio) gene, wdr81, associated with cerebellar ataxia, mental retardation and dysequilibrium syndrome (CAMRQ)

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      Author(s)
      Doldur-Balli, F.
      Ozel, M. N.
      Gulsuner, S.
      Tekinay, A. B.
      Ozcelik, T.
      Konu, O.
      Adams, M. M.
      Date
      2015
      Source Title
      BMC Neuroscience
      Print ISSN
      1471-2202
      Publisher
      BioMed Central Ltd.
      Volume
      16
      Issue
      1
      Language
      English
      Type
      Article
      Item Usage Stats
      266
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      198
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      Abstract
      Background: WDR81 (WD repeat-containing protein 81) is associated with cerebellar ataxia, mental retardation and disequilibrium syndrome (CAMRQ2, [MIM 610185]). Human and mouse studies suggest that it might be a gene of importance during neurodevelopment. This study aimed at fully characterizing the structure of the wdr81 transcript, detecting the possible transcript variants and revealing its expression profile in zebrafish, a powerful model organism for studying development and disease. Results: As expected in human and mouse orthologous proteins, zebrafish wdr81 is predicted to possess a BEACH (Beige and Chediak-Higashi) domain, a major facilitator superfamily domain and WD40-repeats, which indicates a conserved function in these species. We observed that zebrafish wdr81 encodes one open reading frame while the transcript has one 5' untranslated region (UTR) and the prediction of the 3' UTR was mainly confirmed along with a detected insertion site in the embryo and adult brain. This insertion site was also found in testis, heart, liver, eye, tail and muscle, however, there was no amplicon in kidney, intestine and gills, which might be the result of possible alternative polyadenylation processes among tissues. The 5 and 18 hpf were critical timepoints of development regarding wdr81 expression. Furthermore, the signal of the RNA probe was stronger in the eye and brain at 18 and 48 hpf, then decreased at 72 hpf. Finally, expression of wdr81 was detected in the adult brain and eye tissues, including but not restricted to photoreceptors of the retina, presumptive Purkinje cells and some neurogenic brains regions. Conclusions: Taken together these data emphasize the importance of this gene during neurodevelopment and a possible role for neuronal proliferation. Our data provide a basis for further studies to fully understand the function of wdr81.
      Keywords
      In situ hybridization
      QRT-PCR
      RACE
      wdr81
      Zebrafish
      3' untranslated region
      5' untranslated region
      Adult
      Animal tissue
      Article
      Brain disease
      Cerebellar ataxia
      Controlled study
      Disequilibrium syndrome
      Embryo
      Eye
      Gene
      Gene expression profiling
      Gene function
      Genetic association
      Genetic manipulation
      Heart
      Liver
      Male
      Mental deficiency
      Muscle
      Nonhuman
      Open reading frame
      Photoreceptor
      Polyadenylation
      Purkinje cell
      RNA probe
      Tail
      Testis
      Wdr81 gene
      Xebra fish
      Permalink
      http://hdl.handle.net/11693/24813
      Published Version (Please cite this version)
      http://dx.doi.org/10.1186/s12868-015-0229-4
      Collections
      • Department of Molecular Biology and Genetics 542
      • Department of Psychology 242
      • Institute of Materials Science and Nanotechnology (UNAM) 2258
      • Nanotechnology Research Center (NANOTAM) 1179
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