p53 codon 72 polymorphism in bladder cancer-No evidence of association with increased risk or invasiveness
Author
Törüner, G. A.
Uçar, A.
Tez, M.
Çetinkaya, M.
Özen, H.
Özçelik, T.
Date
2001Source Title
Urological Research
Print ISSN
0300-5623
Publisher
Springer
Volume
29
Issue
6
Pages
393 - 395
Language
English
Type
ArticleItem Usage Stats
128
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96
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Abstract
We studied the effect of the p53 gene Arg72Pro polymorphism on bladder cancer susceptibility in a case control study of 121 bladder cancer patients and 114 age-sex matched controls to determine whether this polymorphism is a biomarker for the risk and how aggressive the disease is. Genomic DNA was obtained from venous blood samples for genotype determination by PCR and restriction digestion. The genotype frequencies in the patient group were Arg/Arg: 0.3553, Arg/Pro: 0.4711, Pro/Pro: 0.1736, and in the control group Arg/Arg: 0.3684, Arg/Pro: 0.4825, Pro/Pro: 0.1491. The distribution of genotypes between the two groups was not statistically different (χ2 = 0.260, df: 2, P = 0.878). The patient group was subdivided into two groups as superficial bladder cancer (n = 88) and invasive bladder cancer (n = 33), according to the presence of muscle invasion. The distribution of genotypes in the superficial group was Arg/Arg: 0.3409, Arg/Pro: 0.5114, Pro/Pro: 0.1477 and in the invasive group Arg/Arg: 0.3940, Arg/Pro: 0.3636, Pro/Pro: 0.2424. No association was observed with the invasiveness of the tumor (χ2 = 2.542, df: 2, P = 0.281). Stratification of the data by tobacco exposure did not result in a significant difference in genotype frequencies. These data do not support an association between the p53 Arg72Pro polymorphism and bladder cancer.
Keywords
Bladder cancerGenetic susceptibility
TP53 polymorphism
Biological marker
Protein p53
Adult
Aged
Bladder carcinoma
Cancer invasion
Cancer risk
Cancer susceptibility
Cigarette smoking
Controlled study
Disease activity
DNA polymorphism
Female
Gene frequency
Genetic predisposition
Genotype
Histopathology
Human
Human tissue
Major clinical study
Male
Polymerase chain reaction
Priority journal
Risk assessment
Aged, 80 and over
Female
Genetic Predisposition to Disease
Genotype
Humans
Middle Aged
Neoplasm invasiveness
Polymorphism, single nucleotide
Risk factors
Tumor Suppressor Protein p53
Urinary Bladder Neoplasms
Permalink
http://hdl.handle.net/11693/24759Published Version (Please cite this version)
http://dx.doi.org/10.1007/s002400100218Collections
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