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dc.contributor.authorErdemir, T.en_US
dc.contributor.authorBilican, B.en_US
dc.contributor.authorOncel, D.en_US
dc.contributor.authorGoding, C. R.en_US
dc.contributor.authorYavuzer, U.en_US
dc.date.accessioned2016-02-08T10:33:41Z
dc.date.available2016-02-08T10:33:41Z
dc.date.issued2002en_US
dc.identifier.issn0021-9533
dc.identifier.urihttp://hdl.handle.net/11693/24740
dc.description.abstractThe nuclear matrix protein C1D is an activator of the DNA-dependent protein kinase (DNA-PK), which is essential for the repair of DNA double-strand breaks (DSBs) and V(D)J recombination. C1D is phosphorylated very efficiently by DNA-PK, and its mRNA and protein levels are induced upon γ-irradiation, suggesting that C1D may play a role in repair of DSBs in vivo. In an attempt to identify the biological function of C1D, we have employed the yeast two-hybrid system and found that C1D interacts specifically with Translin-associated factor X, TRAX. Although the biological function of TRAX remains unknown, its bipartite nuclear targeting sequences suggest a role for TRAX in the movement of associated proteins, including Translin, into the nucleus. We show that C1D and TRAX interact specifically in both yeast and mammalian cells. Interestingly, however, interaction of these two proteins in mammalian cells only occur following γ-irradiation, raising the possibility of involvement of TRAX in DNA double-strand break repair and providing evidence for biological functions of the nuclear matrix protein C1D and TRAX. Moreover, we show, using fluorescently tagged proteins, that the relative expression levels of TRAX and Translin affect their subcellular localization. These results suggest that one role for C1D may be to regulate TRAX/Translin complex formation.en_US
dc.language.isoEnglishen_US
dc.source.titleJournal of Cell Scienceen_US
dc.subjectC1D geneen_US
dc.subjectDNA-PKen_US
dc.subjectNuclear matrixen_US
dc.subjectTranslinen_US
dc.subjectTRAXen_US
dc.subjectDNA binding proteinen_US
dc.subjectDNA dependent protein kinaseen_US
dc.titleDNA damage-dependent interaction of the nuclear matrix protein C1D with translin-associated factor X (TRAX)en_US
dc.typeArticleen_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.citation.spage207en_US
dc.citation.epage216en_US
dc.citation.volumeNumber115en_US
dc.citation.issueNumber1en_US


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