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dc.contributor.authorAtaol, S.en_US
dc.contributor.authorTezcaner, A.en_US
dc.contributor.authorDuygulu, O.en_US
dc.contributor.authorKeskin, D.en_US
dc.contributor.authorMachin, N. E.en_US
dc.date.accessioned2016-02-08T10:29:11Z
dc.date.available2016-02-08T10:29:11Z
dc.date.issued2015en_US
dc.identifier.issn1388-0764
dc.identifier.urihttp://hdl.handle.net/11693/24425
dc.description.abstractThe present study evaluates the synthesis of biocompatible osteoconductive and osteoinductive nano calcium phosphate (CaP) particles by industrially applied, aerosol-derived flame spray pyrolysis method for biomedical field. Calcium phosphate nanoparticles were produced in a range of calcium-to-phosphorus ratio, (1.20–2.19) in order to analyze the morphology and crystallinity changes, and to test the bioactivity of particles. The characterization results confirmed that nanometer-sized, spherical calcium phosphate particles were produced. The average primary particle size was determined as 23 nm by counting more than 500 particles in TEM pictures. XRD patterns, HRTEM, SAED, and SEM analyses revealed the amorphous nature of the as-prepared nano calcium phosphate particles at low Ca/P ratios. Increases in the specific surface area and crystallinity were observed with the increasing Ca/P ratio. TGA–DTA analysis showed that the thermally stable crystal phases formed after 700 °C. Cell culture studies were conducted with urine-derived stem cells that possess the characteristics of mesenchymal stem cells. Synthesized amorphous nanoparticles did not have cytotoxic effect at 5–50 μg/ml concentration range. Cells treated with the as-prepared nanoparticles had higher alkaline phosphatase (ALP) enzyme activity than control cells, indicating osteogenic differentiation of cells. A slight decrease in ALP activity of cells treated with two highest Ca:P ratios at 50 μg/ml concentration was observed at day 7. The findings suggest that calcium phosphate nanoparticles produced in this work have a potential to be used as biomaterials in biomedical applications. © 2015, Springer Science+Business Media Dordrecht.en_US
dc.language.isoEnglishen_US
dc.source.titleJournal of Nanoparticle Researchen_US
dc.relation.isversionofhttps://doi.org/10.1007/s11051-015-2901-0en_US
dc.subjectCalcium phosphatesen_US
dc.subjectFlame spray pyrolysisen_US
dc.subjectNanobiotechnologyen_US
dc.subjectNanomedicineen_US
dc.subjectNanoparticlesen_US
dc.subjectOsteogenic differentiationen_US
dc.subjectUSCen_US
dc.titleSynthesis and characterization of nanosized calcium phosphates by flame spray pyrolysis, and their effect on osteogenic differentiation of stem cellsen_US
dc.typeArticleen_US
dc.departmentDepartment of Chemistryen_US
dc.citation.spage1en_US
dc.citation.epage14en_US
dc.citation.volumeNumber17en_US
dc.citation.issueNumber2en_US
dc.identifier.doi10.1007/s11051-015-2901-0en_US
dc.publisherSpringeren_US


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