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dc.contributor.authorUzunalli, G.en_US
dc.contributor.authorTumtas, Y.en_US
dc.contributor.authorDelibasi, T.en_US
dc.contributor.authorYasa, O.en_US
dc.contributor.authorMercan, S.en_US
dc.contributor.authorGuler, M. O.en_US
dc.contributor.authorTekinay, A. B.en_US
dc.date.accessioned2016-02-08T10:29:03Z
dc.date.available2016-02-08T10:29:03Z
dc.date.issued2015en_US
dc.identifier.issn1742-7061
dc.identifier.urihttp://hdl.handle.net/11693/24414
dc.description.abstractPancreatic islet transplantation is a promising treatment for type 1 diabetes. However, viability and functionality of the islets after transplantation are limited due to loss of integrity and destruction of blood vessel networks. Thus, it is important to provide a proper mechanically and biologically supportive environment for enhancing both in vitro islet culture and transplantation efficiency. Here, we demonstrate that heparin mimetic peptide amphiphile (HM-PA) nanofibrous network is a promising platform for these purposes. The islets cultured with peptide nanofiber gel containing growth factors exhibited a similar glucose stimulation index as that of the freshly isolated islets even after 7 days. After transplantation of islets to STZ-induced diabetic rats, 28 day-long monitoring displayed that islets that were transplanted in HM-PA nanofiber gels maintained better blood glucose levels at normal levels compared to the only islet transplantation group. In addition, intraperitoneal glucose tolerance test revealed that animals that were transplanted with islets within peptide gels showed a similar pattern with the healthy control group. Histological assessment showed that islets transplanted within peptide nanofiber gels demonstrated better islet integrity due to increased blood vessel density. This work demonstrates that using the HM-PA nanofiber gel platform enhances the islets function and islet transplantation efficiency both in vitro and in vivo.en_US
dc.language.isoEnglishen_US
dc.source.titleActa Biomaterialiaen_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.actbio.2015.04.032en_US
dc.subjectAngiogenesisen_US
dc.subjectHeparinen_US
dc.subjectIslet transplantationen_US
dc.subjectPeptide nanofibersen_US
dc.subjectSelf-assemblyen_US
dc.subjectAmphophileen_US
dc.subjectFibroblast growth factor 2en_US
dc.subjectGlucoseen_US
dc.subjectGrowth factoren_US
dc.subjectHeparin derivativeen_US
dc.subjectNanofiberen_US
dc.subjectVasculotropinen_US
dc.subjectAngiogenesisen_US
dc.subjectAnimal cellen_US
dc.subjectAnimal experimenten_US
dc.subjectAnimal modelen_US
dc.subjectAnimal tissueen_US
dc.subjectArticleen_US
dc.subjectBlood vesselen_US
dc.subjectCell cultureen_US
dc.subjectCell isolationen_US
dc.subjectCell viabilityen_US
dc.subjectControlled studyen_US
dc.subjectCorneaen_US
dc.subjectEndothelium cellen_US
dc.subjectGelen_US
dc.subjectGlucose blood levelen_US
dc.subjectGlucose tolerance testen_US
dc.subjectHistopathologyen_US
dc.subjectIn vitro studyen_US
dc.subjectInsulin dependent diabetes mellitusen_US
dc.subjectInsulin releaseen_US
dc.subjectMaleen_US
dc.subjectNonhumanen_US
dc.subjectPancreas islet transplantationen_US
dc.subjectPriority journalen_US
dc.subjectRaten_US
dc.subjectStreptozotocin-induced diabetes mellitusen_US
dc.subjectAnimaliaen_US
dc.subjectRattusen_US
dc.titleImproving pancreatic islet in vitro functionality and transplantation efficiency by using heparin mimetic peptide nanofiber gelsen_US
dc.typeArticleen_US
dc.citation.spage8en_US
dc.citation.epage18en_US
dc.citation.volumeNumber22en_US
dc.identifier.doi10.1016/j.actbio.2015.04.032en_US
dc.publisherElsevieren_US


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