Improving pancreatic islet in vitro functionality and transplantation efficiency by using heparin mimetic peptide nanofiber gels
Author
Uzunalli, G.
Tumtas, Y.
Delibasi, T.
Yasa, O.
Mercan, S.
Guler, M. O.
Tekinay, A. B.
Date
2015Source Title
Acta Biomaterialia
Print ISSN
1742-7061
Publisher
Elsevier
Volume
22
Pages
8 - 18
Language
English
Type
ArticleItem Usage Stats
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Show full item recordAbstract
Pancreatic islet transplantation is a promising treatment for type 1 diabetes. However, viability and functionality of the islets after transplantation are limited due to loss of integrity and destruction of blood vessel networks. Thus, it is important to provide a proper mechanically and biologically supportive environment for enhancing both in vitro islet culture and transplantation efficiency. Here, we demonstrate that heparin mimetic peptide amphiphile (HM-PA) nanofibrous network is a promising platform for these purposes. The islets cultured with peptide nanofiber gel containing growth factors exhibited a similar glucose stimulation index as that of the freshly isolated islets even after 7 days. After transplantation of islets to STZ-induced diabetic rats, 28 day-long monitoring displayed that islets that were transplanted in HM-PA nanofiber gels maintained better blood glucose levels at normal levels compared to the only islet transplantation group. In addition, intraperitoneal glucose tolerance test revealed that animals that were transplanted with islets within peptide gels showed a similar pattern with the healthy control group. Histological assessment showed that islets transplanted within peptide nanofiber gels demonstrated better islet integrity due to increased blood vessel density. This work demonstrates that using the HM-PA nanofiber gel platform enhances the islets function and islet transplantation efficiency both in vitro and in vivo.
Keywords
AngiogenesisHeparin
Islet transplantation
Peptide nanofibers
Self-assembly
Amphophile
Fibroblast growth factor 2
Glucose
Growth factor
Heparin derivative
Nanofiber
Vasculotropin
Angiogenesis
Animal cell
Animal experiment
Animal model
Animal tissue
Article
Blood vessel
Cell culture
Cell isolation
Cell viability
Controlled study
Cornea
Endothelium cell
Gel
Glucose blood level
Glucose tolerance test
Histopathology
In vitro study
Insulin dependent diabetes mellitus
Insulin release
Male
Nonhuman
Pancreas islet transplantation
Priority journal
Rat
Streptozotocin-induced diabetes mellitus
Animalia
Rattus