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      Improving pancreatic islet in vitro functionality and transplantation efficiency by using heparin mimetic peptide nanofiber gels

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      Author
      Uzunalli, G.
      Tumtas, Y.
      Delibasi, T.
      Yasa, O.
      Mercan, S.
      Guler, M. O.
      Tekinay, A. B.
      Date
      2015
      Source Title
      Acta Biomaterialia
      Print ISSN
      1742-7061
      Publisher
      Elsevier
      Volume
      22
      Pages
      8 - 18
      Language
      English
      Type
      Article
      Item Usage Stats
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      Abstract
      Pancreatic islet transplantation is a promising treatment for type 1 diabetes. However, viability and functionality of the islets after transplantation are limited due to loss of integrity and destruction of blood vessel networks. Thus, it is important to provide a proper mechanically and biologically supportive environment for enhancing both in vitro islet culture and transplantation efficiency. Here, we demonstrate that heparin mimetic peptide amphiphile (HM-PA) nanofibrous network is a promising platform for these purposes. The islets cultured with peptide nanofiber gel containing growth factors exhibited a similar glucose stimulation index as that of the freshly isolated islets even after 7 days. After transplantation of islets to STZ-induced diabetic rats, 28 day-long monitoring displayed that islets that were transplanted in HM-PA nanofiber gels maintained better blood glucose levels at normal levels compared to the only islet transplantation group. In addition, intraperitoneal glucose tolerance test revealed that animals that were transplanted with islets within peptide gels showed a similar pattern with the healthy control group. Histological assessment showed that islets transplanted within peptide nanofiber gels demonstrated better islet integrity due to increased blood vessel density. This work demonstrates that using the HM-PA nanofiber gel platform enhances the islets function and islet transplantation efficiency both in vitro and in vivo.
      Keywords
      Angiogenesis
      Heparin
      Islet transplantation
      Peptide nanofibers
      Self-assembly
      Amphophile
      Fibroblast growth factor 2
      Glucose
      Growth factor
      Heparin derivative
      Nanofiber
      Vasculotropin
      Angiogenesis
      Animal cell
      Animal experiment
      Animal model
      Animal tissue
      Article
      Blood vessel
      Cell culture
      Cell isolation
      Cell viability
      Controlled study
      Cornea
      Endothelium cell
      Gel
      Glucose blood level
      Glucose tolerance test
      Histopathology
      In vitro study
      Insulin dependent diabetes mellitus
      Insulin release
      Male
      Nonhuman
      Pancreas islet transplantation
      Priority journal
      Rat
      Streptozotocin-induced diabetes mellitus
      Animalia
      Rattus
      Permalink
      http://hdl.handle.net/11693/24414
      Published Version (Please cite this version)
      http://dx.doi.org/10.1016/j.actbio.2015.04.032
      Collections
      • Institute of Materials Science and Nanotechnology 1589
      • Nanotechnology Research Center 873

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