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      Improving pancreatic islet in vitro functionality and transplantation efficiency by using heparin mimetic peptide nanofiber gels

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      Author(s)
      Uzunalli, Gözde
      Tumtas, Yasin
      Delibasi, T.
      Yasa, Oncay
      Mercan, S.
      Güler, Mustafa O.
      Tekinay, Ayse B.
      Date
      2015
      Source Title
      Acta Biomaterialia
      Print ISSN
      1742-7061
      Publisher
      Elsevier
      Volume
      22
      Pages
      8 - 18
      Language
      English
      Type
      Article
      Item Usage Stats
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      Abstract
      Pancreatic islet transplantation is a promising treatment for type 1 diabetes. However, viability and functionality of the islets after transplantation are limited due to loss of integrity and destruction of blood vessel networks. Thus, it is important to provide a proper mechanically and biologically supportive environment for enhancing both in vitro islet culture and transplantation efficiency. Here, we demonstrate that heparin mimetic peptide amphiphile (HM-PA) nanofibrous network is a promising platform for these purposes. The islets cultured with peptide nanofiber gel containing growth factors exhibited a similar glucose stimulation index as that of the freshly isolated islets even after 7 days. After transplantation of islets to STZ-induced diabetic rats, 28 day-long monitoring displayed that islets that were transplanted in HM-PA nanofiber gels maintained better blood glucose levels at normal levels compared to the only islet transplantation group. In addition, intraperitoneal glucose tolerance test revealed that animals that were transplanted with islets within peptide gels showed a similar pattern with the healthy control group. Histological assessment showed that islets transplanted within peptide nanofiber gels demonstrated better islet integrity due to increased blood vessel density. This work demonstrates that using the HM-PA nanofiber gel platform enhances the islets function and islet transplantation efficiency both in vitro and in vivo.
      Keywords
      Heparin
      Peptide nanofibers
      Self-assembly
      Islet transplantation
      Angiogenesis
      Permalink
      http://hdl.handle.net/11693/24414
      Published Version (Please cite this version)
      http://dx.doi.org/10.1016/j.actbio.2015.04.032
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      • Aysel Sabuncu Brain Research Center (BAM) 249
      • Institute of Materials Science and Nanotechnology (UNAM) 2258
      • Nanotechnology Research Center (NANOTAM) 1179
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