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dc.contributor.authorVrana, E.en_US
dc.contributor.authorBuilles, N.en_US
dc.contributor.authorHindie, M.en_US
dc.contributor.authorDamour O.en_US
dc.contributor.authorAydinli, A.en_US
dc.contributor.authorHasirci, V.en_US
dc.date.accessioned2016-02-08T10:10:25Z
dc.date.available2016-02-08T10:10:25Z
dc.date.issued2008en_US
dc.identifier.issn1549-3296
dc.identifier.urihttp://hdl.handle.net/11693/23216
dc.description.abstractCorneal stroma is a very complex structure, composed of 200 lamellae of oriented collagen fibers. This highly complex nature of cornea is known to be important for its transparency and mechanical integrity. Thus, an artificial cornea design has to take into account this complex structure. In this study, behavior of human corneal keratocytes on collagen films patterned with parallel channels was investigated. Keratocytes proliferated well on films and reached confluency after 7 days in the incubation medium. Nearly all of the cells responded to the patterns and were aligned in contrast to the cells on unpatterned surfaces. Collagen type I and keratan sulfate secreted by keratocytes on patterned films appeared to be aligned in the direction of the patterns. The films showed an intermediate degradation over the course of a month. On the whole, transparency of the films increased with degradation and decreased by the presence of the cells. The decrease was, however, low and transparency level was maintained on the patterned films while on the unpatterned films a sharp decrease in transparency was followed by an improvement. This was due to the more organized distribution of cells and the oriented secretion of extracellular matrix molecules on patterned collagen films. Thus, these results suggest that application of contact guidance in cornea tissue engineering may facilitate the remodeling process, hence decrease the rehabilitation period.en_US
dc.language.isoEnglishen_US
dc.source.titleJournal of Biomedical Materials Research - Part Aen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/jbm.a.31442en_US
dc.subjectCollagenen_US
dc.subjectCorneaen_US
dc.subjectMicropatterningen_US
dc.subjectTissue engineeringen_US
dc.subjectTransparencyen_US
dc.subjectArtificial organsen_US
dc.subjectCellsen_US
dc.subjectCollagenen_US
dc.subjectTransparencyen_US
dc.subjectCorneaen_US
dc.subjectHuman corneal keratocytesen_US
dc.subjectMicropatterningen_US
dc.subjectTissue engineeringen_US
dc.subjectCollagen type 1en_US
dc.subjectKeratan sulfateen_US
dc.subjectCell proliferationen_US
dc.subjectControlled studyen_US
dc.subjectCorneaen_US
dc.subjectCornea cellen_US
dc.subjectCornea stromaen_US
dc.subjectCross linkingen_US
dc.subjectHuman cellen_US
dc.subjectScanning electron microscopeen_US
dc.subjectTissue cultureen_US
dc.subjectTissue engineeringen_US
dc.subjectUltraviolet spectrophotometryen_US
dc.subjectAdulten_US
dc.subjectAnimalsen_US
dc.titleContact guidance enhances the quality of a tissue engineered corneal stromaen_US
dc.typeArticleen_US
dc.departmentDepartment of Physicsen_US
dc.citation.spage454en_US
dc.citation.epage463en_US
dc.citation.volumeNumber84en_US
dc.citation.issueNumber2en_US
dc.identifier.doi10.1002/jbm.a.31442en_US
dc.publisherJohn Wiley & Sons, Inc.en_US


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