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      • Department of Molecular Biology and Genetics
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      Strain-and region-specific gene expression profiles in mouse brain in response to chronic nicotine treatment

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      Author(s)
      Wang, J.
      Gutala, R.
      Hwang, Y. Y.
      Kim J. -M.
      Konu, O.
      Ma, J. Z.
      Li, M. D.
      Date
      2008
      Source Title
      Genes, Brain and Behavior
      Print ISSN
      1601-1848
      Publisher
      Wiley-Blackwell Publishing
      Volume
      7
      Issue
      1
      Pages
      78 - 87
      Language
      English
      Type
      Article
      Item Usage Stats
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      154
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      Abstract
      A pathway-focused complementary DNA microarray and gene ontology analysis were used to investigate gene expression profiles in the amygdala, hippocampus, nucleus accumbens, prefrontal cortex (PFC) and ventral tegmental area of C3H/HeJ and C57BL/6J mice receiving nicotine in drinking water (100 μg/ml in 2% saccharin for 2 weeks). A balanced experimental design and rigorous statistical analysis have led to the identification of 3.5-22.1% and 4.1-14.3% of the 638 sequence-verified genes as significantly modulated in the aforementioned brain regions of the C3H/HeJ and C57BL/6J strains, respectively. Comparisons of differential expression among brain tissues showed that only a small number of genes were altered in multiple brain regions, suggesting presence of a brain region-specific transcriptional response to nicotine. Subsequent principal component analysis and Expression Analysis Systematic Explorer analysis showed significant enrichment of biological processes both in C3H/HeJ and C57BL/6J mice, i.e. cell cycle/proliferation, organogenesis and transmission of nerve impulse. Finally, we verified the observed changes in expression using real-time reverse transcriptase polymerase chain reaction for six representative genes in the PFC region, providing an independent replication of our microarray results. Together, this report represents the first comprehensive gene expression profiling investigation of the changes caused by nicotine in brain tissues of the two mouse strains known to exhibit differential behavioral and physiological responses to nicotine.
      Keywords
      Brain regions
      C3H/HeJ mice
      C57BL/6J mice
      Microarray
      Nicotine
      PCA
      Drinking water
      Saccharin
      Amygdaloid nucleus
      Animal experiment
      Animal tissue
      Article
      Behavior
      Brain
      Brain region
      Brain tissue
      Cell cycle
      Cell proliferation
      Controlled study
      DNA microarray
      Gene expression profiling
      Gene identification
      Hippocampus
      Mouse
      Nerve conduction
      Nonhuman
      Nucleus accumbens
      Organogenesis
      Prefrontal cortex
      Principal component analysis
      Priority journal
      Real time polymerase chain reaction
      Statistical analysis
      Ventral tegmentum
      Animals
      Behavior, animal
      Cell division
      Gene expression
      Gene expression profiling
      Male
      Mice
      Mice, Inbred C3H
      Mice, Inbred C57BL
      Neurons
      Oligonucleotide array sequence analysis
      Organogenesis
      Polymerase Chain Reaction
      Reverse transcriptase polymerase chain reaction
      Species specificity
      Synaptic transmission
      Transcription, genetic
      Permalink
      http://hdl.handle.net/11693/23215
      Published Version (Please cite this version)
      http://dx.doi.org/10.1111/j.1601-183X.2007.00328.x
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