Strain-and region-specific gene expression profiles in mouse brain in response to chronic nicotine treatment
Date
2008Source Title
Genes, Brain and Behavior
Print ISSN
1601-1848
Publisher
Wiley-Blackwell Publishing
Volume
7
Issue
1
Pages
78 - 87
Language
English
Type
ArticleItem Usage Stats
229
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154
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Abstract
A pathway-focused complementary DNA microarray and gene ontology analysis were used to investigate gene expression profiles in the amygdala, hippocampus, nucleus accumbens, prefrontal cortex (PFC) and ventral tegmental area of C3H/HeJ and C57BL/6J mice receiving nicotine in drinking water (100 μg/ml in 2% saccharin for 2 weeks). A balanced experimental design and rigorous statistical analysis have led to the identification of 3.5-22.1% and 4.1-14.3% of the 638 sequence-verified genes as significantly modulated in the aforementioned brain regions of the C3H/HeJ and C57BL/6J strains, respectively. Comparisons of differential expression among brain tissues showed that only a small number of genes were altered in multiple brain regions, suggesting presence of a brain region-specific transcriptional response to nicotine. Subsequent principal component analysis and Expression Analysis Systematic Explorer analysis showed significant enrichment of biological processes both in C3H/HeJ and C57BL/6J mice, i.e. cell cycle/proliferation, organogenesis and transmission of nerve impulse. Finally, we verified the observed changes in expression using real-time reverse transcriptase polymerase chain reaction for six representative genes in the PFC region, providing an independent replication of our microarray results. Together, this report represents the first comprehensive gene expression profiling investigation of the changes caused by nicotine in brain tissues of the two mouse strains known to exhibit differential behavioral and physiological responses to nicotine.
Keywords
Brain regionsC3H/HeJ mice
C57BL/6J mice
Microarray
Nicotine
PCA
Drinking water
Saccharin
Amygdaloid nucleus
Animal experiment
Animal tissue
Article
Behavior
Brain
Brain region
Brain tissue
Cell cycle
Cell proliferation
Controlled study
DNA microarray
Gene expression profiling
Gene identification
Hippocampus
Mouse
Nerve conduction
Nonhuman
Nucleus accumbens
Organogenesis
Prefrontal cortex
Principal component analysis
Priority journal
Real time polymerase chain reaction
Statistical analysis
Ventral tegmentum
Animals
Behavior, animal
Cell division
Gene expression
Gene expression profiling
Male
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Neurons
Oligonucleotide array sequence analysis
Organogenesis
Polymerase Chain Reaction
Reverse transcriptase polymerase chain reaction
Species specificity
Synaptic transmission
Transcription, genetic
Permalink
http://hdl.handle.net/11693/23215Published Version (Please cite this version)
http://dx.doi.org/10.1111/j.1601-183X.2007.00328.xCollections
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