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dc.contributor.authorBüyüktanir, O.en_US
dc.contributor.authorYildirim, T.en_US
dc.contributor.authorYakicier, C.en_US
dc.contributor.authorGenç, O.en_US
dc.contributor.authorYurdusev, N.en_US
dc.date.accessioned2016-02-08T10:09:11Z
dc.date.available2016-02-08T10:09:11Z
dc.date.issued2008en_US
dc.identifier.issn0378-1135
dc.identifier.urihttp://hdl.handle.net/11693/23118
dc.description.abstractMycoplasma gallisepticum is the primary agent of chronic respiratory disease causing important economic losses in the poultry industry. Serological monitoring is essential to maintain mycoplasma-free breeder flocks and often complicated by the cross-reactions between different mycoplasma species. To overcome serological cross-reactions, a large fragment of the M. gallisepticum PvpA cytadhesin, species-specific surface-exposed protein, was produced in E. coli as a recombinant protein (rPvpA336) and used as a potential diagnostic antigen. The rPvpA336 protein possesses 336 mycoplasma-specific amino acids with relative molecular weight of 44 kDa. A deletion region of 37 amino acids was identified when compared to the wild-type PvpA protein. Immunoreactivity of the rPvpA336 protein has been demonstrated by Western blot analysis with M. gallisepticum-positive and -negative chicken sera. Furthermore, an enzymatic rapid immunofiltration assay (ERIFA) prototype based on the rPvpA336 protein has been developed and its species-specific detection capability has been demonstrated by using M. gallisepticum and/or M. synoviae-positive and -negative chicken sera. In addition to its species-specificity, the ERIFA prototype presents certain advantages such as rapidity, field-applicability and cost-effectiveness. Therefore, these advantages would make the prototype a species-specific rapid diagnostic tool of choice in the field and limited laboratory conditions for screening M. gallisepticum infections. © 2007 Elsevier B.V. All rights reserved.en_US
dc.language.isoEnglishen_US
dc.source.titleVeterinary Microbiologyen_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.vetmic.2007.11.028en_US
dc.subjectChickenen_US
dc.subjectMycoplasma gallisepticumen_US
dc.subjectRecombinant PvpAen_US
dc.subjectSerodiagnostic prototypeen_US
dc.subjectPvpa proteinen_US
dc.subjectRecombinant proteinen_US
dc.subjectUnclassified drugen_US
dc.subjectAnimal experimenten_US
dc.subjectRecombinant Proteinsen_US
dc.titleA recombinant PvpA protein-based diagnostic prototype for rapid screening of chicken mycoplasma gallisepticum infectionsen_US
dc.typeArticleen_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.citation.spage139en_US
dc.citation.epage149en_US
dc.citation.volumeNumber129en_US
dc.citation.issueNumber1-2en_US
dc.identifier.doi10.1016/j.vetmic.2007.11.028en_US
dc.publisherElsevieren_US


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