Chemical and topographical modification of PHBV surface to promote osteoblast alignment and confinement
Date
2008Source Title
Journal of Biomedical Materials Research - Part A
Print ISSN
1549-3296
Publisher
John Wiley & Sons, Inc.
Volume
85A
Issue
4
Pages
1001 - 1010
Language
English
Type
ArticleItem Usage Stats
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Abstract
Proper cell attachment and distribution, and thus stronger association in vivo between a bone implant and native tissue will improve the success of the implant. In this study, the aim was to achieve promotion of attachment and uniform distribution of rat mesenchymal stem cell-derived osteoblasts by introducing chemical and topographical cues on poly(3-hydroxybutyrate-co-3- hydroxyvalerate) (PHBV) film surfaces. As the chemical cues, either alkaline phosphatase was covalently immobilized on the film surface to induce deposition of calcium phosphate minerals or fibrinogen was adsorbed to improve cell adhesion. Microgrooves and micropits were introduced on the film surface by negative replication of micropatterned Si wafers. Both chemical cues improved cell attachment and even distribution on the PHBV films, but Fb was more effective especially when combined with the micropatterns. Cell alignment (<10° deviation angle) parallel to chemically modified microgrooves (1, 3, or 8 μm groove width) and on 10 μm-thick Fb lines printed on the unpatterned films was achieved. The cells on unpatterned and 5 μm-deep micropitted films were distributed and oriented randomly. Results of this study proved that microtopographies on PHBV can improve osseointegration when combined with chemical cues, and that microgrooves and cell adhesive protein lines on PHBV can guide selective osteoblast adhesion and alignment.
Keywords
Bone tissue engineeringMicropatterned films
Osteoblasts
PHBV
Photolithography
Bone
Cell culture
Implants (surgical)
Photolithography
Silicon wafers
Cell attachment
Film surface
Micropatterned films
Osteoblasts
Alkaline phosphatase
Calcium phosphate
Fibrinogen
Poly (3 hydroxybutyric acid)
Silicon
Valeric acid derivative
Animal cell
Bone regeneration
Cell adhesion
Cellular distribution
Chemical cue
Chemical modification
Controlled study
Mesenchyme cell
Nonhuman
Osteoblast
Rat
Surface property
Tissue engineering
Topography
Acridine orange
Animals
Cell adhesion
Cell count
Cells, cultured
Osteoblasts
Polyesters
Polystyrenes
Rats, Sprague-Dawley
Surface Properties