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      Chemical and topographical modification of PHBV surface to promote osteoblast alignment and confinement

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      Author(s)
      Kenar, H.
      Kocabas, A.
      Aydınlı, Atilla
      Hasirci, V.
      Date
      2008
      Source Title
      Journal of Biomedical Materials Research - Part A
      Print ISSN
      1549-3296
      Publisher
      John Wiley & Sons, Inc.
      Volume
      85A
      Issue
      4
      Pages
      1001 - 1010
      Language
      English
      Type
      Article
      Item Usage Stats
      222
      views
      200
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      Abstract
      Proper cell attachment and distribution, and thus stronger association in vivo between a bone implant and native tissue will improve the success of the implant. In this study, the aim was to achieve promotion of attachment and uniform distribution of rat mesenchymal stem cell-derived osteoblasts by introducing chemical and topographical cues on poly(3-hydroxybutyrate-co-3- hydroxyvalerate) (PHBV) film surfaces. As the chemical cues, either alkaline phosphatase was covalently immobilized on the film surface to induce deposition of calcium phosphate minerals or fibrinogen was adsorbed to improve cell adhesion. Microgrooves and micropits were introduced on the film surface by negative replication of micropatterned Si wafers. Both chemical cues improved cell attachment and even distribution on the PHBV films, but Fb was more effective especially when combined with the micropatterns. Cell alignment (<10° deviation angle) parallel to chemically modified microgrooves (1, 3, or 8 μm groove width) and on 10 μm-thick Fb lines printed on the unpatterned films was achieved. The cells on unpatterned and 5 μm-deep micropitted films were distributed and oriented randomly. Results of this study proved that microtopographies on PHBV can improve osseointegration when combined with chemical cues, and that microgrooves and cell adhesive protein lines on PHBV can guide selective osteoblast adhesion and alignment.
      Keywords
      Bone tissue engineering
      Micropatterned films
      Osteoblasts
      PHBV
      Photolithography
      Bone
      Cell culture
      Implants (surgical)
      Photolithography
      Silicon wafers
      Cell attachment
      Film surface
      Micropatterned films
      Osteoblasts
      Alkaline phosphatase
      Calcium phosphate
      Fibrinogen
      Poly (3 hydroxybutyric acid)
      Silicon
      Valeric acid derivative
      Animal cell
      Bone regeneration
      Cell adhesion
      Cellular distribution
      Chemical cue
      Chemical modification
      Controlled study
      Mesenchyme cell
      Nonhuman
      Osteoblast
      Rat
      Surface property
      Tissue engineering
      Topography
      Acridine orange
      Animals
      Cell adhesion
      Cell count
      Cells, cultured
      Osteoblasts
      Polyesters
      Polystyrenes
      Rats, Sprague-Dawley
      Surface Properties
      Permalink
      http://hdl.handle.net/11693/23088
      Published Version (Please cite this version)
      http://dx.doi.org/10.1002/jbm.a.31638
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      • Department of Physics 2484
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