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dc.contributor.authorPlagnol V.en_US
dc.contributor.authorUz, E.en_US
dc.contributor.authorWallace, C.en_US
dc.contributor.authorStevens H.en_US
dc.contributor.authorClayton, D.en_US
dc.contributor.authorOzcelik, T.en_US
dc.contributor.authorTodd J.A.en_US
dc.date.accessioned2016-02-08T10:08:06Z
dc.date.available2016-02-08T10:08:06Z
dc.date.issued2008en_US
dc.identifier.issn19326203
dc.identifier.urihttp://hdl.handle.net/11693/23035
dc.description.abstractLymphoblastoid cell lines (LCL) are being actively and extensively used to examine the expression of specific genes and (genome-wide expression profiles, including allele specific expression assays. However, it has recently been shown that approximately 10% of human genes exhibit random patterns of monoallelic expression within single clones of LCLs. Consequently allelic imbalance studies could be significantly compromised if bulk populations of donor cells are clonal, or near clonal. Here, using X chromosome inactivation as a readout, we confirm and quantify widespread near monoclonality in two independent sets of cell lines. Consequently, we recommend where possible the use of bulk, non cell line, ex vivo cells for allele specific expression assays. © 2008 Plagnol et al.en_US
dc.language.isoEnglishen_US
dc.source.titlePLoS ONEen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pone.0002966en_US
dc.subjectadulten_US
dc.subjectalleleen_US
dc.subjectallelic imbalanceen_US
dc.subjectarticleen_US
dc.subjectchilden_US
dc.subjectclonal variationen_US
dc.subjectcontrolled studyen_US
dc.subjectfemaleen_US
dc.subjectgene expression profilingen_US
dc.subjectgenome analysisen_US
dc.subjecthumanen_US
dc.subjecthuman cellen_US
dc.subjectinsulin dependent diabetes mellitusen_US
dc.subjectlymphoblastoid cell lineen_US
dc.subjectmajor clinical studyen_US
dc.subjectnewbornen_US
dc.subjectX chromosome inactivationen_US
dc.subjectblood cellen_US
dc.subjectcell cloneen_US
dc.subjectcell cultureen_US
dc.subjectcell lineen_US
dc.subjectcytologyen_US
dc.subjectgene expressionen_US
dc.subjectgeneticsen_US
dc.subjectimmunologyen_US
dc.subjectlymphocyteen_US
dc.subjectphysiologyen_US
dc.subjectquantitative trait locusen_US
dc.subjectreference valueen_US
dc.subjectsingle nucleotide polymorphismen_US
dc.subjectRNAen_US
dc.subjectBlood Cellsen_US
dc.subjectCell Lineen_US
dc.subjectCells, Cultureden_US
dc.subjectClone Cellsen_US
dc.subjectFemaleen_US
dc.subjectGene Expressionen_US
dc.subjectHumansen_US
dc.subjectLymphocytesen_US
dc.subjectPolymorphism, Single Nucleotideen_US
dc.subjectQuantitative Trait Locien_US
dc.subjectReference Valuesen_US
dc.subjectRNAen_US
dc.titleExtreme clonality in lymphoblastoid cell lines with implications for allele specific expression analysesen_US
dc.typeArticleen_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.citation.volumeNumber3en_US
dc.citation.issueNumber8en_US
dc.identifier.doi10.1371/journal.pone.0002966en_US


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