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dc.contributor.authorTincer G.en_US
dc.contributor.authorBayyurt, B.en_US
dc.contributor.authorArıca, Y.M.en_US
dc.contributor.authorGürsel İ.en_US
dc.date.accessioned2016-02-08T10:07:59Z
dc.date.available2016-02-08T10:07:59Z
dc.date.issued2015en_US
dc.identifier.issn1301109X
dc.identifier.urihttp://hdl.handle.net/11693/23025
dc.description.abstractObjectives: Chitosan is a widely used vaccine or anti-cancer delivery vehicle. In this study, we investigated the immunomodulatory effect of chitosan/pIC nanocomplexes on mouse immune cells. Materials and methods: Proliferative and cytotoxic features of chitosan were tested via CCK-8 assay on RAW 264. 7. IL-1β production was assessed via ELISA from PEC supernatants. TNF-α, and NO induction from chitosan treated RAW cells detected by ELISA and Griess assay, respectively. mRNA message levels of TLRs and cytokines on macrophages in response to chitosan/pIC nanocomplex treatments were evaluated by RT-PCR. Results: Results revealed that chitosan is non-toxic to cells, however, proliferative capacities of macrophages were reduced by chitosan administration. Mouse PECs treated with chitosan, led to NLRP3 dependent inflammasome activation as evidenced by dose-dependent IL-1β secretion. Chitosan/pIC nanocomplexes did not improve immunostimulatory action of pIC on RAW cells, since TNF-α and NO productions remained unaltered. Expression levels of several TLRs, CXCL-16 and IFN-α messages from mouse splenocytes were down regulated in response to chitosan/pIC nanocomplex treatment. Conclusion: Our results revealed that chitosan is an anti-proliferative and inflammasome triggering macromolecule on immune cells. Utilization of chitosan as a carrier system is of concern for immunotherapeutic applications. © 2015 Turkish Journal of Immunology.en_US
dc.language.isoEnglishen_US
dc.source.titleTurkish Journal of Immunologyen_US
dc.relation.isversionofhttp://dx.doi.org/10.5606/tji.2015.329en_US
dc.subjectBiomaterialen_US
dc.subjectChitosanen_US
dc.subjectDouble-stranded ribonucleic aciden_US
dc.subjectInnate immunityen_US
dc.subjectPolysaccharideen_US
dc.subjectToll like receptoren_US
dc.subjectalpha interferonen_US
dc.subjectchitosanen_US
dc.subjectcryopyrinen_US
dc.subjectCXCL16 chemokineen_US
dc.subjectgamma interferon inducible protein 10en_US
dc.subjectinflammasomeen_US
dc.subjectinterleukin 1betaen_US
dc.subjectmacrophage inflammatory protein 3alphaen_US
dc.subjectnitric oxideen_US
dc.subjectpolyinosinic polycytidylic aciden_US
dc.subjectpolysaccharideen_US
dc.subjecttoll like receptoren_US
dc.subjecttoll like receptor 1en_US
dc.subjecttoll like receptor 2en_US
dc.subjecttoll like receptor 3en_US
dc.subjecttoll like receptor 4en_US
dc.subjecttoll like receptor 6en_US
dc.subjecttoll like receptor 7en_US
dc.subjecttoll like receptor 9en_US
dc.subjecttumor necrosis factor alphaen_US
dc.subjectanimal cellen_US
dc.subjectArticleen_US
dc.subjectcell proliferationen_US
dc.subjectcontrolled studyen_US
dc.subjectcytokine productionen_US
dc.subjectcytokine releaseen_US
dc.subjectcytotoxicityen_US
dc.subjectdown regulationen_US
dc.subjectgene expressionen_US
dc.subjectimmunomodulationen_US
dc.subjectmouseen_US
dc.subjectnonhumanen_US
dc.subjectprotein expressionen_US
dc.subjectprotein inductionen_US
dc.titleChitosan polysaccharide suppress toll like receptor dependent immune responseen_US
dc.title.alternativeÇitosan polisakkaridi toll benzeri reseptöre bağlı bağışıklık yanıtını baskılaren_US
dc.typeArticleen_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.citation.spage15en_US
dc.citation.epage20en_US
dc.citation.volumeNumber3en_US
dc.citation.issueNumber1en_US
dc.identifier.doi10.5606/tji.2015.329en_US
dc.publisherTurkish Society of Immunologyen_US


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