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      • Department of Molecular Biology and Genetics
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      Differential expression patterns of metastasis suppressor proteins in basal cell carcinoma

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      Author(s)
      Bozdogan, O.
      Yulug, I. G.
      Vargel, I.
      Cavusoglu, T.
      Karabulut, A. A.
      Karahan, G.
      Sayar, N.
      Date
      2015
      Source Title
      International Journal of Dermatology
      Print ISSN
      0011-9059
      Publisher
      Wiley-Blackwell Publishing Ltd.
      Volume
      54
      Issue
      8
      Pages
      905 - 915
      Language
      English
      Type
      Article
      Item Usage Stats
      203
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      305
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      Abstract
      Background: Basal cell carcinomas (BCCs) are common malignant skin tumors. Despite having a significant invasion capacity, they metastasize only rarely. Our aim in this study was to detect the expression patterns of the NM23-H1, NDRG1, E-cadherin, RHOGDI2, CD82/KAI1, MKK4, and AKAP12 metastasis suppressor proteins in BCCs. Methods: A total of 96 BCC and 10 normal skin samples were included for the immunohistochemical study. Eleven frozen BCC samples were also studied by quantitative real time polymerase chain reaction (qRT-PCR) to detect the gene expression profile. Results: NM23-H1 was strongly and diffusely expressed in all types of BCC. Significant cytoplasmic expression of NDRG1 and E-cadherin was also detected. However, AKAP12 and CD82/KAI1 expression was significantly decreased. The expressions of the other proteins were somewhere between the two extremes. Similarly, qRT-PCR analysis showed down-regulation of AKAP12 and up-regulation of NM23-H1 and NDRG1 in BCC. Morphologically aggressive BCCs showed significantly higher cytoplasmic NDRG1 expression scores and lower CD82/KAI1 scores than non-aggressive BCCs. Conclusion: The relatively preserved levels of NM23-H1, NDRG1, and E-cadherin proteins may have a positive effect on the non-metastasizing features of these tumors.
      Keywords
      AKAP12 protein
      CD82 antigen
      Metastasis suppressor protein
      Mitogen activated protein kinase kinase 4
      N myc downstream regulated I protein
      Nucleoside diphosphate kinase A
      Protein
      Rho guanine nucleotide dissociation inhibitor 2
      Unclassified drug
      Uvomorulin
      Aged
      Article
      Basal cell carcinoma
      Controlled study
      Down regulation
      Female
      Gene expression profiling
      Human
      Human tissue
      Immunohistochemistry
      Major clinical study
      Male
      Protein expression
      Reverse transcription polymerase chain reaction
      Upregulation
      Permalink
      http://hdl.handle.net/11693/22891
      Published Version (Please cite this version)
      http://dx.doi.org/10.1111/ijd.12581
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      • Department of Molecular Biology and Genetics 542
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