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      • Department of Molecular Biology and Genetics
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      Nuclear exclusion of p33ING1b tumor suppressor protein: explored in HCC cells using a new highly specific antibody

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      Author(s)
      Sayan, B.
      Emre, N. C. T.
      Irmak, M. B.
      Ozturk, M.
      Cetin Atalay, R.
      Date
      2009
      Source Title
      Hybridoma
      Print ISSN
      1554-0014
      Publisher
      Mary Ann Liebert, Inc
      Volume
      28
      Issue
      1
      Pages
      1 - 6
      Language
      English
      Type
      Article
      Item Usage Stats
      200
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      202
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      Abstract
      Mouse monoclonal antibodies (MAb) were generated against p33ING1b tumor suppressor protein. 15B9 MAb was highly specific in recognizing a single protein band of ∼33 kDa endogenous p33ING1b protein from HCC cell lines and normal liver tissue by Western blot analysis and by immunoprecipitation. Although p33ING1b mutations are rarely observed in cancer, differential subcellular distribution and nuclear exclusion of p33ING1b were reported in different cancer types. Therefore we analyzed the expression and subcellular localization of p33ING1b in HCC cell lines using 15B9 MAb. So far, p33ING1b mutations or differential subcellular localization are not reported in HCC. In this study, by indirect immunofluorescence using MAb 15B9, we demonstrate that nuclear localization of p33ING1b was highly correlated with well-differentiated HCC cell lines whereas poorly differentiated HCC cells have nuclear exclusion of the protein. Moreover no association was observed between differential subcellular localization of p33ING1b and p53 mutation status of HCC cell lines. Hence our newly produced MAb 15B9 can be used for studying cellular activities of p33ING1b under normal and cancerous conditions. © Copyright 2009, Mary Ann Liebert, Inc.
      Keywords
      Monoclonal antibody
      Protein p53
      Tumor suppressor protein
      Unclassified drug
      Animal cell
      Animal experiment
      Animal tissue
      Antibody production
      Cancer cell culture
      Cell activity
      Cell differentiation
      Cellular distribution
      Human cell
      Immunofluorescence
      Liver cell carcinoma
      Mouse
      Mutation
      Permalink
      http://hdl.handle.net/11693/22845
      Published Version (Please cite this version)
      http://dx.doi.org/10.1089/hyb.2008.0058
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