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      Antiangiogenic response after 70% hepatectomy and its relationship with hepatic regeneration and angiogenesis in rats

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      Author
      Dogrul, A.B.
      Colakoglu, T.
      Kosemehmetoglu, K.
      Birben, E.
      Yaman, E.
      Gedikoglu G.
      Abbasoglu O.
      Date
      2010
      Source Title
      Surgery
      Print ISSN
      0039-6060
      Volume
      147
      Issue
      2
      Pages
      288 - 294
      Language
      English
      Type
      Article
      Item Usage Stats
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      Abstract
      Background: The aim of this study was to evaluate the antiangiogenic response and its relation to regeneration and angiogenesis after 70% hepatectomy in a rat model. Methods: Sixty-four Wistar albino rats were included in the study. Animals were allocated into 8 groups (n = 8). After a 70% hepatectomy, liver regeneration, angiogenesis, and antiangiogenic response were evaluated in the remnant liver on days 0, 1, 2, 3, 5, 7, 10, and 14. Regeneration and angiogenesis were determined with immunoreactivity to proliferating cell nuclear antigen and vascular endothelial growth factor. Antiangiogenic response was evaluated by detecting collagen 18 m RNA with reverse transcriptase polymerase chain reaction. Results: We showed that liver regeneration peaked at day 1, whereas angiogenesis in the periportal and perisinusoidal areas reached their peak values on days 3 and 7, respectively. Both regeneration and angiogenic activity around perisinusoidal hepatocytes returned to basal activity on the day 10. Antiangiogenic response first appeared on day 5, reached a peak on day 10, and returned to basal values on day 14. Conclusion: Collagen18 mRNA expression is present in the normal liver during the regenerative process. We suggest that the stimulus that causes the cessation of regeneration process may come from hepatocytes, and collagen 18 produced by hepatocytes may modulate this event by inhibiting the angiogenesis. © 2010 Mosby, Inc. All rights reserved.
      Keywords
      cell nucleus antigen
      collagen
      collagen 18
      messenger RNA
      unclassified drug
      vasculotropin
      angiogenesis
      animal cell
      animal experiment
      animal tissue
      article
      cell activity
      cell stimulation
      controlled study
      experimental model
      experimental surgery
      female
      gene expression
      immunoreactivity
      liver cell
      liver regeneration
      liver resection
      liver sinusoid
      nonhuman
      priority journal
      rat
      reverse transcription polymerase chain reaction
      stimulus
      treatment response
      Wistar rat
      Angiogenesis Inhibitors
      Animals
      Collagen Type XVIII
      Female
      Hepatectomy
      Hepatocytes
      Liver
      Liver Regeneration
      Neovascularization, Physiologic
      Proliferating Cell Nuclear Antigen
      Rats
      Rats, Wistar
      Reverse Transcriptase Polymerase Chain Reaction
      Vascular Endothelial Growth Factor A
      Permalink
      http://hdl.handle.net/11693/22444
      Published Version (Please cite this version)
      http://dx.doi.org/10.1016/j.surg.2009.10.015
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      • Department of Molecular Biology and Genetics 426
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