Antiangiogenic response after 70% hepatectomy and its relationship with hepatic regeneration and angiogenesis in rats
Author
Dogrul, A.B.
Colakoglu, T.
Kosemehmetoglu, K.
Birben, E.
Yaman, E.
Gedikoglu G.
Abbasoglu O.
Date
2010Source Title
Surgery
Print ISSN
0039-6060
Volume
147
Issue
2
Pages
288 - 294
Language
English
Type
ArticleItem Usage Stats
136
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views
59
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Abstract
Background: The aim of this study was to evaluate the antiangiogenic response and its relation to regeneration and angiogenesis after 70% hepatectomy in a rat model. Methods: Sixty-four Wistar albino rats were included in the study. Animals were allocated into 8 groups (n = 8). After a 70% hepatectomy, liver regeneration, angiogenesis, and antiangiogenic response were evaluated in the remnant liver on days 0, 1, 2, 3, 5, 7, 10, and 14. Regeneration and angiogenesis were determined with immunoreactivity to proliferating cell nuclear antigen and vascular endothelial growth factor. Antiangiogenic response was evaluated by detecting collagen 18 m RNA with reverse transcriptase polymerase chain reaction. Results: We showed that liver regeneration peaked at day 1, whereas angiogenesis in the periportal and perisinusoidal areas reached their peak values on days 3 and 7, respectively. Both regeneration and angiogenic activity around perisinusoidal hepatocytes returned to basal activity on the day 10. Antiangiogenic response first appeared on day 5, reached a peak on day 10, and returned to basal values on day 14. Conclusion: Collagen18 mRNA expression is present in the normal liver during the regenerative process. We suggest that the stimulus that causes the cessation of regeneration process may come from hepatocytes, and collagen 18 produced by hepatocytes may modulate this event by inhibiting the angiogenesis. © 2010 Mosby, Inc. All rights reserved.
Keywords
cell nucleus antigencollagen
collagen 18
messenger RNA
unclassified drug
vasculotropin
angiogenesis
animal cell
animal experiment
animal tissue
article
cell activity
cell stimulation
controlled study
experimental model
experimental surgery
female
gene expression
immunoreactivity
liver cell
liver regeneration
liver resection
liver sinusoid
nonhuman
priority journal
rat
reverse transcription polymerase chain reaction
stimulus
treatment response
Wistar rat
Angiogenesis Inhibitors
Animals
Collagen Type XVIII
Female
Hepatectomy
Hepatocytes
Liver
Liver Regeneration
Neovascularization, Physiologic
Proliferating Cell Nuclear Antigen
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Vascular Endothelial Growth Factor A
Permalink
http://hdl.handle.net/11693/22444Published Version (Please cite this version)
http://dx.doi.org/10.1016/j.surg.2009.10.015Collections
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