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      Portable microfluidic integrated plasmonic platform for pathogen detection

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      Author
      Tokel, O.
      Yildiz, U. H.
      Inci, F.
      Durmus, N. G.
      Ekiz, O. O.
      Turker, B.
      Cetin, C.
      Rao, S.
      Sridhar, K.
      Natarajan, N.
      Shafiee, H.
      Dana, A.
      Demirci, U.
      Date
      2015
      Source Title
      Scientific Reports
      Print ISSN
      2045-2322
      Publisher
      Nature Publishing Group
      Volume
      5
      Pages
      1 - 9
      Language
      English
      Type
      Article
      Item Usage Stats
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      84
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      Abstract
      Timely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods, such as enzyme linked immunosorbent assay (ELISA), culturing or polymerase chain reaction (PCR) require long assay times, and complex and expensive instruments, which are not adaptable to point-of-care (POC) needs at resource-constrained as well as primary care settings. Therefore, there is an unmet need to develop simple, rapid, and accurate methods for detection of pathogens at the POC. Here, we present a portable, multiplex, inexpensive microfluidic-integrated surface plasmon resonance (SPR) platform that detects and quantifies bacteria, i.e., Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) rapidly. The platform presented reliable capture and detection of E. coli at concentrations ranging from ∼105 to 3.2 × 107 CFUs/mL in phosphate buffered saline (PBS) and peritoneal dialysis (PD) fluid. The multiplexing and specificity capability of the platform was also tested with S. aureus samples. The presented platform technology could potentially be applicable to capture and detect other pathogens at the POC and primary care settings. © 2015, Nature Publishing Group. All rights reserved.
      Keywords
      Biotechnology
      Health care
      Nanobiotechnology
      Permalink
      http://hdl.handle.net/11693/22236
      Published Version (Please cite this version)
      https://doi.org/10.1038/srep09152
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      • Institute of Materials Science and Nanotechnology (UNAM) 1872
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